HSV-1 IgM ELISA Kit (Herpes Simplex Virus 1  HSV-1)

Full Name: HSV-1 IgM ELISA Kit (Herpes Simplex Virus 1  HSV-1)
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.06 U/ml


Infections with are due to herpes simplex virus are commonly referred to as herpes and these can be caused by either HSV-1 or HSV-2. HSV-1 is a highly contagious infection, which is acquired during childhood and the infection can be lifelong. The vast majority of infections are found to be oral herpes (also known as orolabial, oral-facial and oral-labial herpes), a proportion of HSV-1 infections are also genital herpes (infections in the genital and anal area).

Infected persons will usually experience a burning, tingling or itching sensation around their mouth, which in majority of cases is before the appearance of sores.  One of the “gold standard” for diagnosing infections is through the isolation of the virus in tissue culture, following by either western blotting or indirect immunofluorescence.


Human HSV-1 IgM ELISA kit can be used for measuring in-vitro concentrations of human IgM antibodies against herpes simplex virus-1 (HSV-1 IgM) in plasma or serum. This assay has a minimum analytical sensitivity limit of 1.06 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Herpes simplex virus-1 antigen coated microtiter strips.
  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).
  • Sample Diluent.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.


The minimum detection sensitivity level of human IgM antibodies to herpes simplex virus-1 (HSV-1 IgM) using current HSV-1 IgM ELISA kit was 1.06 U/ml. The dynamic range for this assay is 1.0 – 100.0 U/ml.


– Intra-Assay-Precision: 8.6 %
– Inter-Assay-Precision: 9.4 %
– Inter-Lot-Precision: 5.6 – 11.0 %
– Analytical Sensitivity: 1.06 U/mL
– Recovery: 90 – 97 %
– Linearity: 68 – 121 %
– Cross-Reactivity: No cross-reactivity to Mumps, Measles or Varicella.
– Interferences: No interferences to triglycerides up to 5.0 mg/mL, bilirubin up to 0.3 mg/mL or hemoglobin up to 8.0 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %


  1. Analysis of the IgM and IgG antibody response against herpes simplex virus type 1 (HSV-1) structural and nonstructural proteins. J Med Virol. (1987) 23 (2): 135-50. Kühn J.E., et al.
  2. Evaluation of commercial herpes simplex virus-IgG and IgM enzyme immunoassays. J Virol Methods. (2014) 199: 29-34. Liermann K., et al.
  3. Comparison of several ELISA tests for detecting the presence of IgG and IgM against herpes simplex viruses. Microbios. (2000) 103 (405): 127-32. Gutiérrez J., et al.
  4. Reactivated herpes simplex infection increases the risk of Alzheimer’s disease. Alzheimers Dement. (2015) 11 (6): 593-9. Lövheim H., et al.


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford