Rat GRO/CINC-2 Beta ELISA Kit

INTENDED USE

Rat GRO/CINC-2 beta ELISA kit is a method for analysing quantitative concentrations of GRO/CINC-2-beta (GRO/CINC-2b, GRO/CINC-2β) in serum and cell culture supernatant. This assay has a minimum sensitivity detection limit of 7.0 pg/ml.

BACKGROUND

GRO/CINC-1 (also known as cytokine-induced neutrophil chemo attractant 1) was first identified in purified media that was conditioned using IL-1 beta stimulated rat kidney epithelial cells. It belongs to the CXC chemokines family and there are three different family members: CINC-2a, CINC-2b and CINC-3 (MIP-2). These display protein sequence homology between 63-67% and are the rat counterpart to human GRO.

Grow Related Oncogene (GRO) is a member of the IL-8 family of polypeptides and has three isoforms (alpha, beta and gamma), these share sequence identity of 68%, 71% and 68% respectively. The expression of GRO was found to be upregulated in human breast cancer cells compared to normal cells. The role of GRO in inflammatory response is to induce chemotactic migration and transmigration of neutrophils and monocytes. The binding to the CXC chemokine receptor 3 (CXCR3) causes neutrophils to leave the blood vessels, cross into tissue and release cytokines that cause chromatin remodelling in mast cells.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti- rat GRO/CINC-2β Rabbit IgG.
• Standard.
• Labelled Antibody.
• EIA Buffer.
• Solution For Labelled Antibody.
• TMB Chromogen.
• Wash Buffer (40x Concentrated).
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of GRO/CINC-2b (GRO/CINC-2-beta) using this rat GRO/CINC-2 beta ELISA kit was approximately 7.0 pg/ml. The dynamic range for this assay is 9.38 – 600.0 pg/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 91.1 – 113.2%
– Added Recovery Assay: 92.7 – 102.1%
– Intra – Assay: 2.9 – 7.5%
– Inter – Assay: 1.7 – 6.6%
– Specificity: Rat GRO/CINC-2 beta (100%)
– Sensitivity: 7.0 pg/mL

REFERENCES

1. Neutrophil chemoattractant 2 beta regulates expression of the Reg gene in injured gastric mucosa in rats. Gastroenterology. (2000) 119 (6): 1610-22. Kazumori H., et al.
2. Expression of CINC-2 beta is related to the state of differentiation of alveolar epithelial cells. Am J Respir Cell Mol Biol. (2005) 33 (5): 505-12. Nishina K., et al.
3. Gene structure, cDNA cloning, and expression of the rat cytokine-induced neutrophil chemoattractant-2 (CINC-2) gene. Cytokine. (1998) 10 (3): 169-74. Shibata F., et al.

ADDITIONAL INFORMATION

• Full Name: Rat GRO/CINC-2 Beta ELISA Kit
• Reactivity: Rat
• Sample Type: Serum, Cell Culture Media Supernatant
• Sensitivity: 7.0 pg/ml

OTHER RELATED ELISA KITS

• GRO/CINC-2 Alpha ELISA Kit (Rat)
• GRO/CINC-3 ELISA Kit (Rat)
• GRO/CINC-1 ELISA Kit (Rat)

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