Rat GRO/CINC-3 ELISA Kit

INTENDED USE

Rat GRO/CINC-3 ELISA kit is designed for measuring quantitative levels of both native and recombinant GRO/CINC-3 using rat samples of serum, plasma-EDTA and cell culture supernatant. This assay has a minimum sensitivity detection limit of 0.51 pg/ml.

BACKGROUND

Rat fibroblasts and alveolar macrophages that have been activated by cytokines express cytokine-induced neutrophil chemo attractant 3. Based on its DNA and protein sequences, it is also referred to as rat MIP-2, CXCL1 and GRO beta. It is expressed by bone marrow cells in response to TNF alpha activation, where it increases the recruitment of neutrophils to the site of inflammation.

GRO/CINC-3 is a member of the alpha CXC chemokines subfamily and the protein sequence of rat CINC-3 is found to display 88% identity with murine MIP-2. It has been identified to possess a potent chemo tactic factor for rat neutrophil both in vivo and in vitro. CINC-3 and CINC-4 are structurally similar proteins with a similar domain structure. The two proteins differ only in their C-terminal regions, wherein CINC-3 contains a tyrosine kinase domain at its C terminus and lacks an important region for protein binding, while CINC4 has an SH2 domain which is absent from the former.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti-rat GRO/CINC-3 Rabbit IgG.
• Labelled Antibody Concentrated.
• EIA Buffer.
• Standard.
• Solution For Labelled Antibody.
• TMB Chromogen.
• Wash Buffer (40x Concentrated).
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of GRO/CINC-3 using this rat GRO/CINC-3 ELISA kit was approximately 0.51 pg/ml. The dynamic assay range for this kit is 4.69 – 300.0 pg/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 80.5 – 113.9%
– Added Recovery Assay: 78.0 – 95.3%
– Intra – Assay: 4.8 – 5.9%
– Inter – Assay: 4.2 – 4.6%
– Specificity: Rat GRO/CINC-3 (100%)
– Sensitivity: 0.51 pg/mL

REFERENCES

1. Recombinant production and biological properties of rat cytokine-induced neutrophil chemoattractants, GRO/CINC-2 alpha, CINC-2 beta and CINC-3. Eur J Biochem. (1995) 231 (2): 306-11. Shibata F., et al.
2. Lipopolysaccharide Upregulates the Expression of CINC-3 and LIX in Primary NG2 Cells. Neurochem Res. (2016) 41 (6): 1448-57. Li Y., et al.
3. Contribution of cytokine-induced neutrophil chemoattractant CINC-2 and CINC-3 to neutrophil recruitment in lipopolysaccharide-induced inflammation in rats. Inflamm Res. (2001) 50 (10): 503-8. Takano K. and Nakagawa H.
4. Expression of cytokine-induced neutrophil chemoattractant in rat lungs following an intratracheal instillation of micron-sized nickel oxide nanoparticle agglomerates. Toxicol Ind Health. (2014) 30 (9): 851-60. Morimoto Y., et al.

ADDITIONAL INFORMATION

• Full Name: Rat GRO/CINC-3 ELISA Kit
• Reactivity: Rat
• Sample Type: EDTA-Plasma, Serum, Cell Culture Supernatant
• Sensitivity: 0.51 pg/ml

OTHER RELATED ELISA KITS

• GRO/CINC-2 Alpha ELISA Kit (Rat)
• GRO/CINC-1 ELISA Kit (Rat)
• Growth Hormone ELISA Kit (Rat/Mouse)