Mouse GIP active ELISA kit is a procedure intended for detecting in vitro quantitative levels of mouse active form gastric inhibitory polypeptide (GIP active form, GIP [1-42]) using plasma samples. This assay has a minimum sensitivity detection limit of 1.5 pg/ml.
Gastric inhibitory polypeptide (GIP) is derived from a 153 amino proprotein that belongs to the secretin family. It has a major role in the stimulation of insulin secretion, however, it is also a weak inhibitor of gastric acid secretion and this helps to protect small intestines from stomach acid damage. Recently, there is evidence to indicate that GIP may be a vital player in bone remodelling, it was discovered that mice which had GIP receptor production disrupted resulted in profound alteration of bone microarchitecture. There are reports to suggest absence of GIP receptors display’s a correlation to resistance to obesity in knockout mice studies.
MOUSE GIP ACTIVE ELISA KIT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Antibody Coated Plate: Mouse anti GIP (1-42) monoclonal antibody.
- Standard (Lyophilized).
- HRP Labelled Antibody Solution.
- Enzyme Substrate Solution (TMB).
- Stopping Solution.
- Buffer Solution.
- Washing Solution (Concentrated).
- Adhesive Foil.
The minimum sensitivity detection limit of gastric inhibitory polypeptide (active form GIP, GIP [1-42]) using current mouse GIP active ELISA kit was approximately 1.5 pg/ml. The dynamic range for this assay is 7.8 – 500.0 pg/ml.
– Mouse GIP Active (1-42): ELISA
– Analytical Recovery: 91.7 – 106.7%
– Dilution Test: 102.6 – 110.5%
– Cross-Reactivity: Mouse GIP (1-42) (100%)
– Intra-Assay CV: 5.8 – 6.0%
– Inter-Assay CV: 1.6 – 3.3%
– Assay Range: 7.8 – 500 pg/mL.
- Vaccination against GIP for the treatment of obesity. PLoS One. (2008) 3 (9): e3163. Fulurija A., et al.
- Active immunization against (Pro(3)) GIP improves metabolic status in high-fat-fed mice. Diabetes Obes Metab. (2010) 12 (9): 744-51. Montgomery I.A., et al.
- On the physiology of GIP and GLP-1. Horm Metab Res. (2004) 36 (11-12): 747-54. Review. Holst J.J.
- Novel insulin/GIP co-producing cell lines provide unexpected insights into Gut K-cell function in vivo. J Cell Physiol. (2002) 192 (3): 339-50. Ramshur E.B., et al.
- GIP as a potential therapeutic agent? Horm Metab Res. (2004) 36 (11-12): 859-66. Review. Meier J.J. and Nauck M.A.
- Full Name: GIP Active Form ELISA Kit (Mouse)
- Reactivity: Mouse
- Sample Type: EDTA-Plasma
- Sensitivity: 1.5 pg/ml