Mouse GIP Active ELISA Kit (Gastric Inhibitory Polypeptide)

Full Name: Mouse GIP Active ELISA Kit (Gastric Inhibitory Polypeptide)
Reactivity: Mouse
Sample Type: EDTA-Plasma
Sensitivity: 1.5 pg/ml

BACKGROUND

The secretin family includes the 153-amino acid proprotein known as gastric inhibitory polypeptide (GIP). GIP is created by endocrine cells in the small intestine and then released into the blood. As an orexigenic hormone, it increases appetite and encourages the storage of fat. These belong to the same family which also includes the well-known hormone ghrelin. Enteroendocrine cells, which predominately exist in the ileum, jejunum, and duodenum, produce GIPs. They are well known to play a crucial role in controlling hunger.

GIP protein promotes the release of insulin among other crucial functions. It reduces gastric acid production, which helps to prevent small intestine damage from stomach acid. The absence of GIP-receptors directly corresponds with resistance to obesity, according to studies employing knockout mice and recent research suggesting that GIP may play essential roles in bone remodelling. Given that the GH/IGF-1 axis controls the expression of this gene, it has also been recognised as a potential regulator of bone maturation. GIP knockouts are more susceptible to obesity because they are less responsive to the GH and IGF-1 axis.

INTENDED USE

Mouse GIP active ELISA kit is a procedure intended for detecting in vitro quantitative levels of gastric inhibitory polypeptide (GIP active form, GIP [1-42]) using mouse plasma samples. This assay has a minimum sensitivity detection limit of 1.5 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Antibody Coated Plate: Mouse anti GIP (1-42) monoclonal antibody.
• Standard (Lyophilized).
• HRP Labelled Antibody Solution.
• Enzyme Substrate Solution (TMB).
• Stopping Solution.
• Buffer Solution.
• Washing Solution (Concentrated).
• Adhesive Foil.

SENSITIVITY

The minimum sensitivity detection limit of gastric inhibitory polypeptide (active form GIP, GIP [1-42]) using current mouse GIP active ELISA kit was approximately 1.5 pg/ml. The dynamic range for this assay is 7.8 – 500.0 pg/ml.

ASSAY CHARACTERISTICS

– Analytical Recovery: 91.7 – 106.7%
– Dilution Test: 102.6 – 110.5%
– Cross-Reactivity: Mouse GIP (1-42) (100%)
– Intra-Assay CV: 5.8 – 6.0%
– Inter-Assay CV: 1.6 – 3.3%
– Assay Range: 7.8 – 500 pg/mL.

REFERENCES

1. Vaccination against GIP for the treatment of obesity. PLoS One. (2008) 3 (9): e3163. Fulurija A., et al.
2. Active immunization against (Pro(3)) GIP improves metabolic status in high-fat-fed mice. Diabetes Obes Metab. (2010) 12 (9): 744-51. Montgomery I.A., et al.
3. On the physiology of GIP and GLP-1. Horm Metab Res. (2004) 36 (11-12): 747-54. Review. Holst J.J.
4. Novel insulin/GIP co-producing cell lines provide unexpected insights into Gut K-cell function in vivo. J Cell Physiol. (2002) 192 (3): 339-50. Ramshur E.B., et al.
5. GIP as a potential therapeutic agent? Horm Metab Res. (2004) 36 (11-12): 859-66. Review. Meier J.J. and Nauck M.A.

OTHER RELATED ELISA KITS

• GIP Active Form ELISA Kit (Rat)
• Insulin ELISA Kit (Rat)
• Leptin ELISA Kit (Mouse)