Human free progesterone saliva ELISA kit is an accurate in vitro quantitative procedure for detecting unconjugated free progesterone (pregn-4-ene-3 and 20-dione) in human saliva samples. This assay has a minimum sensitivity detection limit of 5.0 pg/ml.
Progesterone is also known as 20-dione and pregn-4-ene-3, is a female sex hormone which is a C21 steroid that contains a keto-group at position C3 and has a double bond at C4 and C5. It is synthesised predominately in the corpus luteum of the ovaries, during pregnancy in the placenta and in very small amounts in the adrenal cortex or the testes.
Progesterone is a vital endocrine hormone that is essential in the regulation of the menstrual cycle. Following ovulation, progesterone is secreted via the corpus luteum that can develop from the ovulated follicle into the ovaries. A major task for this hormone is to prepare the genital organs of women for implantation and then to help in maintaining the pregnancy. Progesterone is also responsible for introducing the secretory phase of the endometrium, stimulating the growth of mammary tissue, suppressing the contractions of the uterus, in addition to effecting metabolism and the endocrine system in women.
The following salivary progesterone ELISA kit is an essential tool which is frequently used to detect levels of active human free progesterone. During circulation majority of progesterone is found to be bound to the corticoid binding globulin (CBG or transcortin), sex hormone binding globulin (SHBG) and to albumin. Only a small amount between 1-2% is circulating as free progesterone. The free hormone is the only one that is representing the active form during endocrine regulation. Also, it must be noted that free progesterone is released in equal amounts in saliva. In the salivary glands it is presumed that an enzymatic metabolisation of portions of this hormone occurs. This vital property makes sampling of this hormone using saliva very attractive because of the many benefits it offers over using serum or plasma.
Progesterone is secreted into the circulation in a pulsating way and the levels present in circulation are reflected in its concentrations in saliva. Therefore, measuring progesterone using saliva can be useful for monitoring the menstrual cycle in order to assess the time of ovulation and to determine the function of corpus luteum which is important during the early stages of pregnancy. Also, in order to perform a convenient hormone profile it is recommended to carry out many repeated samples due to the pulsating way progesterone is secreted into the circulation. This requirement makes saliva sampling (which represents the concentration of active free progesterone) an ideal method to carry out this analysis.
FREE PROGESTERONE SALIVA ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Coated with rabbit polyclonal anti-progesterone antibody.
- Calibrator 0.
- Calibrators 1-6: Concentrations 10, 30, 100, 300, 1000, 5000 pg/ml, made up from human progesterone.
- Control Low And Control High.
- Enzyme Conjugate: Consisting of progesterone conjugated to HRP.
- Wash Buffer Concentrate (10x).
- TMB Substrate Solution.
- Stop Solution.
The minimum sensitivity detection limit of human free progesterone using current salivary progesterone ELISA kit was approximately 5.0 pg/ml. The dynamic range for this assay is 10.0 – 5,000.0 pg/ml.
– Free Salivary Progesterone (Free Progesterone Saliva): ELISA
– Analytical Sensitivity: 5.0 pg/ml
– Specificity (Cross Reactivity): Highly specific for prgesterone.
– Assay Dynamic Range: 0 – 5000 pg/ml.
– Intra-Assay Variation: 5.96 – 9.63%
– Inter-Assay Variation: 8.6 – 10.1%
– Recovery: 78.6 – 105.5%
– Linearity: 82.1 – 114.2%
– Drug Interference: None
- Salivary progesterone excellently reflects free and total progesterone in plasma during pregnancy. Clin Chem. (1989) 35 (1): 168-72. Meulenberg P.M. and Hofman J.A.
- Stimulated and unstimulated saliva progesterone in menopausal women with oral dryness feeling. Clin Oral Investig. (2011) 15 (6): 859-62. Mirzaii-Dizgah I. and Agha-Hosseini F.
- A direct radioimmunoassay for free progesterone in saliva. J Med Assoc Thai. (1994) 77 (3): 138-47. Vienravi V., et al.
- Determination of free progesterone in an ultrafiltrate of saliva collected in situ. Clin Chem. (1990) 36 (8 Pt 1): 1488-93. Schramm W., et al.
- Full Name: Free Progesterone Saliva ELISA Kit
- Reactivity: Human
- Sample Type: Saliva
- Sensitivity: 5.0 pg/ml