Estrone ELISA Kit
INTENDED USE
Human estrone ELISA kit is a reliable quantitative procedure for measuring estrone in human serum and plasma. This assay has a minimum sensitivity detection limit of 8.1 pg/ml.
BACKGROUND
Estrone is mainly biosynthesised from cholesterol, the reaction involving this process occurs predominately in the gonads and the adipose tissues. Once produced it is then secreted from these tissues to the rest of the body. Around 80% is found to be bound to albumin in circulation, 16% bound to SHBG and the remaining 4% unbound or freely circulating. It functions by diffusing through cell membrane where it binds and actives the nuclear-estrogen receptors, these receptors are known be present in the reproductive tract, hypothalamus, pituitary, bone, liver and breast.
Estrone elevates the synthesis of SHBG (sex hormone binding globulin) and TBG (thyroid binding globulin). There are many bioassay data which are indicating that the estrogenic activity can be much lower when compared to that of estradiol. Androstenedione is primarily responsible for its production. In men, postmenopausal women and prepubertal children the predominant portion can be derived from conversion of peripheral tissues. Also, more than 50% can be secreted by the ovaries in premenopausal women.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiterwells: Break apart strips containing wells that are coated with estrone antigen.
- Standards 0-6: Concentration 0-2400 pg/mL. The standards are calibrated against certified reference material code E-075 (Cerilliant).
- Control Low and Control High: Contains human estrone.
- Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase.
- Substrate Solution: Tetramethylbenzidine (TMB).
- Stop Solution: 0.5 M H2SO4, Avoid contact with the stop solution. It may cause skin irritations and burns.
- Wash Solution (40x Concentrated).
SENSITIVITY
The minimum sensitivity detection limit of estrone using current estrone ELISA kit was approximately 8.1 pg/ml. The dynamic range for this assay is 15.0 – 2,400.0 ng/ml.
ASSAY CHARACTERISTICS
– Assay Dynamic Range: 8.1 – 2400 pg/ml
– Specificity (Cross Reactivity): Estradiol (1.19%), Estrone 3-sulfate (0.44%), Cortisone (0.39%), Ethisterone (0.39%), Estrone 3-β-D-glucoronide (0.35%), Hydrocortisone (0.30%), Estriol (0.07%), Androstenedione (0.04%), Testosterone (0.03%), DHEA (0.01%), 17-OH-Progesterone (0.01%), Corticosterone (0.01%), Progesterone (< 0.01%).
– Analytical Sensitivity: 8.1 pg/ml
– Intra-Assay Variability: 6.4 – 8.4%
– Inter-Assay Variability: 7.4 – 11.9%
– Inter-Lot Variability: 2.3 – 14.0%
– Recovery: 89.2 – 113.4%
– Linearity: 85.0 – 113.4%
– Drug Interferences: None
– Interfering Substances: None
– High-dose-Hook Effect: None
REFERENCES
- Estrone sulfatase and its inhibitors. Anticancer Agents Med Chem. (2009) 9 (6): 599-612. Review. Aidoo-Gyamfi K., et al.
- Conjugation of estrone glucuronide with human lysozyme. J Immunoassay Immunochem. (2015) 36 (5): 532-46. Cooke D.G and Blackwell L.F.
- Estrone is neuroprotective in rats after traumatic brain injury. J Neurotrauma. (2012) 29 (12): 2209-19. Gatson J.W., et al.
ADDITIONAL INFORMATION
- Full Name: Estrone ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 8.1 pg/ml
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