CXCL10 (IP-10) ELISA Kit

Full Name: CXCL10 (IP-10) ELISA Kit
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 4.688 pg/ml


CXCL10 (IP-10) is a monomeric structure that exhibits a typical chemokine fold consisting of a three-stranded β sheet overlaid by an α helix with a number of the receptor binding residues located in the associated loops stabilized by the disulfide bonds. IP-10 is secreted by leukocytes and tissue cells and functions as a chemoattractant, mainly for lymphocytes. After binding to its receptor CXCR3, it evokes a range of inflammatory responses which are key features in cardiovascular disease (CVD).

CXCL10 is expressed by monocytes, endothelial cells, keratinocytes and fibroblasts. It is produced by neutrophils in reaction to a combined stimulus of IFN- and TNF or bacterial lipopolysaccharide. As neurons also express it, a main ligand for CXCR33, it has been suggested that neuronal CXCL10 may have a role in recruitment and homing of glial cells during embryogenesis.


Human CXCL10 (IP-10) ELISA kit can be used for detecting amounts of interferon gamma induced 10kDa (CXCL10, IP-10, gIP-10, mob-1) present is plasma, serum, biological fluids and tissue homogenate samples.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-cxcl10 antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.


For this ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml.
Reactivity: Human
Sensitivity: 4.688 pg/ml
Range: 7.813 – 500 pg/ml
Principle: Sandwich
Application: Research Use Only.


– Specificity: Highly specific for CXCL10, no cross reactivity or interference between CXCL10 and analogues was detected.
– Recovery: Serum (88 – 104%), EDTA Plasma (92 – 104%), Heparin Plasma (85 – 98%).
– Linearity: Serum (88 – 102%), EDTA Plasma (86 – 98%), Heparin Plasma (83 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.


  1. Chemokines beyond chemo-attraction: CXCL10 and its significant role in cancer and autoimmunity. (2018) 109: 24-28. Karin N. and Razon H.
  2. CXCL10/CXCR3 signaling contributes to an inflammatory microenvironment and its blockade enhances progression of murine pancreatic precancerous lesions. (2021) 10: e60646. Pandey V., et al.
  3. CXCL10/IP-10 in infectious diseases pathogenesis and potential therapeutic implications. Cytokine Growth Factor Rev. (2011) 22 (3): 121-30. Liu M., et al.
  4. The multifaceted functions of CXCL10 in cardiovascular disease. Biomed Res Int. (2014) 2014: 893106. van den Borne P., et al.


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford