Full Name: Chlamydia Trachomatis IgA ELISA Kit
Sample Type: Serum, Plasma
Sensitivity: diagn. >95.0%
Chlamydia trachomatis is a prevalent sexually transmitted bacterial pathogen with a unique two-stage developmental cycle involving infectious elementary bodies and replicative reticulate bodies. The elementary bodies are tiny spherical structures that function like spores, attaching to and entering epithelial cells of the host. After entering a cell, the elementary bodies differentiate into reticulate bodies that proliferate via binary fission inside a membrane-bound inclusion vacuole. The reticulate bodies later revert back to elementary bodies that exit the cell and spread the infection.
C. trachomatis targets columnar epithelial cells within the urethra and reproductive system. It escapes immune defenses by remaining intracellular and blocking fusion of lysosomes with its inclusion vacuole. Through its type III secretion apparatus, C. trachomatis injects effector proteins into the host cell that facilitate infection. Some strains harbor a cryptic plasmid that may encode virulence factors. Inflammation often occurs as immune cells react to infected epithelial cells.
There are multiple C. trachomatis serovars causing distinct diseases. Serovars A-C induce ocular infections including trachoma, the leading preventable cause of blindness globally. Serovars D-K infect the urogenital tract, eliciting nongonococcal urethritis, cervicitis, pelvic inflammatory disease, and infertility. Lymphogranuloma venereum is provoked by serovars L1-L3. Vertical transmission from mother to newborn can result in infantile pneumonia and conjunctivitis. Since genital infections are frequently asymptomatic, routine screening and treatment are vital for disease control.
Human Chlamydia trachomatis IgA ELISA kit is a protocol for measuring in-vitro levels of IgA class antibodies to Chlamydia trachomatis (Chlamydia trachomatis-IgA) using either plasma or serum. This assay has a minimum analytical sensitivity limit to a diagn. >95.0%.
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Chlamydia trachomatis Coated Microplate (IgA).
- IgA Sample Diluent.
- Chlamydia trachomatis anti-IgA Conjugate.
- Positive Control.
- Cut-off Control.
- Negative Control.
- TMB Substrate Solution.
- Washing Buffer (20x Conc.).
- Stop Solution.
The minimum detection sensitivity level of human IgA class antibodies against Chlamydia trachomatis using current Chlamydia trachomatis IgA ELISA kit was to a diagn. >95.0%. The dynamic range for this assay is to a specific cut-off point.
– Cut-Off: 10 U
– Equivocal: 9 – 11 U
– Negative: < 9 U
– Positive: > 11 U
– Diagnostic Specificity: 98.81%
– Diagnostic Sensitivity: 96.55%
– Cross Reactivity: No false-positive discovered.
– Interferences: No Interferences with hemolytic, lipemic or icteric samples are used.
- Serum specific IgA antibody to Chlamydia trachomatis in patients with chlamydial infections detected by ELISA and an immunofluorescence test. J Clin Pathol. (1984) 37 (6): 686-91. Cevenini R., et al.
- Do IgA antibodies to Chlamydia-trachomatis have protective role in humoral immunity: a study in reactive arthritis patients. Microbes Infect. (2015) 17 (11-12): 806-10. Kumar P., et al.
- IgA antibodies to Chlamydia trachomatis and metabolic syndrome. Microbiol Immunol. (2010) 54 (12): 747-9. Sehnem L., et al.
- Prevalence and diagnostic significance of specific IgA and anti-heat shock protein 60 Chlamydia trachomatis-antibodies in subfertile women. Eur J Clin Microbiol Infect Dis. (2014) 33 (5): 761-6. Arsovic A., et al.
- Chlamydia trachomatis and male infertility: chlamydia-IgA antibodies in seminal plasma are C. trachomatis specific and associated with an inflammatory response. J Eur Acad Dermatol Venereol. (1999) 12 (2): 143-52. Ochsendorf F.R., et al.
OTHER RELATED ELISA KITS
- Chlamydia Trachomatis-IgG ELISA Kit
- Chlamydia Trachomatis-IgM ELISA Kit
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