Chitinase-3-Like Protein 1 ELISA Kit (CHI3L1)
INTENDED USE
Human CHI3L1 ELISA kit can measure concentrations of CHI3L1 (Chitinase-3-Like Protein 1, GP-39, ASRT7, CGP-39, YYL-40, YKL-40, Glycoprotein 39) present is plasma, serum, biological fluids or tissue homogenate samples.
INTRODUCTION
CHI3L1, also known as chitinase 3 protein 1 or YKL 40 is a protein found in mammals that has been linked to processes such, as inflammation, cancer development and tissue changes. Interestingly even though it lacks chitinase enzyme activity it still plays a role in these processes. CHI3L1 is released by types of cells like macrophages and cancer cells and its levels have been observed to be higher in diseases associated with inflammation and alterations in the extracellular matrix.
Despite not having activity like chitinase proteins do CHI3L1 interacts with components of the matrix and sends signals inside cells. These signals may be involved in promoting cancer cell growth activating macrophages supporting blood vessel formation (angiogenesis) and influencing how tissues respond to mechanical strain and oxidative stress. However the specific molecular mechanisms through which CHI3L1 exerts its effects are still not fully understood.
In conditions characterized by inflammation and tissue changes such as arthritis, liver fibrosis, atherosclerosis, asthma, inflammatory bowel disease (IBD) and various types of cancer; elevated levels of CHI3L1 have been observed. Measuring circulating levels of CHI3L1 shows promise as a tool for predicting treatment responses and prognosis in cancers as well as chronic inflammatory conditions.
However there are still uncertainties regarding whether increased levels of CHI3L1 drive processes or if they represent a protective response or consequence downstream. In the context of cancer specifically higher levels of CHI3L1 generally correlate with prognoses; however knockout studies suggest that the protein may actually inhibit stages of tumor formation.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti- CHI3L1.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this CHI3L1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml.
Reactivity: Human
Sensitivity: 37.5 pg/ml
Range: 62.5 – 4000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for CHI3L1, no cross reactivity or interference between CHI3L1 and analogues was detected.
– Recovery: Serum (88 – 103%), EDTA Plasma (86 – 103%), Heparin Plasma (85 – 98%).
– Linearity: Serum (89 – 103%), EDTA Plasma (84 – 100%), Heparin Plasma (90 – 95%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%
REFERENCES
- Chitinase-3 like-protein-1 function and its role in diseases. Signal Transduct Target Ther. (2020) 5 (1): 201. Zhao T., et al.
- CHI3L1 in the pathophysiology and diagnosis of liver diseases. Biomed Pharmacother. (2020) 131: 110680. Wang S., et al.
- Chitinase 3 like 1 contributes to the development of pulmonary vascular remodeling in pulmonary hypertension. JCI Insight. (2022) 7 (18): e159578. Sun X., et al.
- Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization. Mol Oncol. (2022) 16 (11): 2214-2234. Yu J.E., et al.
ADDITIONAL INFORMATION
- Full Name: Chitinase-3-Like Protein 1 ELISA Kit (CHI3L1)
- Reactivity: Human
- Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
- Sensitivity: 37.5 pg/ml
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