CTXII ELISA Kit (Cross Linked C-telopeptide of Type II Collagen)

INTENDED USE

Human CTXII ELISA kit can measure concentrations of cross linked C-telopeptide of type II collagen (CTXII, CTX-II) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a sensitivity detection limit of 0.094 ng/ml.

INTRODUCTION

CTXII is a peptide biomarker utilized to assess the turnover and degradation of cartilage. When there is collagen turnover it is produced as a result of the breakdown of type II cartilage collagen. This biomarker plays a role, in providing information about the condition of cartilage tissue and has significant applications in medical research and patient care.

Increased levels in fluids indicate cartilage breakdown, which proves helpful in detecting and monitoring the progression of osteoarthritis and rheumatoid arthritis. Osteoarthritis specifically involves the deterioration of cartilage over time resulting in pain, stiffness and reduced mobility. By analyzing urine or blood serum to measure CTXII levels, early signs of high cartilage turnover can be identified. This enables measures to be taken in order to slow down disease progression through therapies and lifestyle modifications. Consequently CTXII-testing serves as a tool for managing arthritis and promoting health.

In addition to its applications in diagnosing and monitoring arthritis it also exhibits potential as an indicator for tracking recovery progress following cartilage injuries or surgeries. A study demonstrated that CTXII levels gradually decreased over a span of 24 months after implantation surgery on knee injuries involving defects, in the cartilage. This suggests that this biomarker may prove useful in monitoring the maturation process of implanted cartilage cells.

Further research suggests that the development of traumatic osteoarthritis can potentially be predicted by measuring CTXII shortly after experiencing acute knee trauma that involves cartilage damage. CTXII as an indicator of the health and degradation of cartilage has a range of applications, in research and treatment particularly in understanding the progression of joint diseases monitoring therapy responses and assessing tissue regeneration.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- CTXII.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this CTXII ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CTXII, no cross reactivity or interference between CTXII and analogues was detected.
– Recovery: Serum (89 – 96%), EDTA Plasma (85 – 104%), Heparin Plasma (93 – 105%).
– Linearity: Serum (90 – 105%), EDTA Plasma (82 – 100%), Heparin Plasma (84 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Serum CTXii Correlates With Articular Cartilage Degeneration After Anterior Cruciate Ligament Transection or Arthrotomy Followed by Standardized Exercise. Sports Health. (2012) 4 (6): 510-7. Coyle C.H., et al.
2. Achyranthoside D attenuates chondrocyte loss and inflammation in osteoarthritis via targeted regulation of Wnt3a. Phytomedicine. (2023) 111: 154663. Xie W., et al. al.
3. Cathepsin K inhibition reduces CTXII levels and joint pain in the guinea pig model of spontaneous osteoarthritis. Osteoarthritis Cartilage. (2010) 18 (10): 1355-7. McDougall J.J., et al.

ADDITIONAL INFORMATION

• Full Name: CTXII ELISA Kit (Cross Linked C-telopeptide of Type II Collagen)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.094 ng/ml

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