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Chikungunya Virus IgG Capture ELISA Kit

INTENDED USE

Human Chikungunya virus IgG ELISA kit is a method for detecting in-vitro quantitative amounts of IgG class antibodies against Chikungunya virus (Chikungunya IgG) in human plasma or serum. This assay has a minimum analytical sensitivity limit to a diagn. >90%.

BACKGROUND

Chikungunya is a mosquito-borne viral disease that belongs to the alphavirus genus Togaviridae family. The virus can be transmitted from one human to another by the bites from an infected female mosquitoes. Some of the common symptoms are severe joint pain, muscle pain, fever, fatigue, rash and headache. Chikungunya disease is found to shares some clinical signs with are very similar to zika and dengue. At present it has been identified in over 60 countries that include Europe, America, Africa and Asia. There are several methods which have been used for its diagnosis, for example serological test like ELISA have been routinely used to confirm the presence of either IgG or IgM anti-chikungunya antibodies. Also, it have been identified that the levels of IgM antibody are at their highest between 3 to 5 weeks following the onset of illness and this can persist for up to 2 months.

CHIKUNGUNYA IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Chikungunya Coated Microplate (IgG).
  • Sample Diluent.
  • Stop Solution.
  • Washing Solution (20x Conc.).
  • Chikungunya Solution (Lyophilised).
  • Biotinylated Chikungunya Antibody.
  • Streptavidin Conjugate.
  • TMB Substrate.
  • Positive Control.
  • Negative Control.

SENSITIVITY

The minimum detection sensitivity level of IgG class antibodies against Chikungunya virus (Chikungunya-IgG) using current human Chikungunya IgG ELISA kit was to a a diagn. >90%. The dynamic range for this assay is to a specific cut-off point.

ASSAY CHARACTERSTICS

– Chikungunya Virus ELISA: IgG
– Cut-Off: 10 NTU
– Grey Zone: 9-11 NTU
– Negative: < 9 NTU
– Positive: > 11 NTU
– Diagnostic Sensitivity is > 90%
– Diagnostic Specificity is > 90%
– Cross Reactivity: No cross reactivity to Dengue virus, Tick born encephalitis, CMV, EBV and Helicobacter pylori.
– Interferences: No interferences were observed when adding trigycerides, bilirubin and haemoglobin in an excess to the sample.

REFERENCES

  1. Analysis of antibody response (IgM, IgG, IgG3) to Chikungunya virus using panel of peptides derived from envelope protein for serodiagnosis. Clin Chem Lab Med. (2014) 52 (2): 297-307. Verma P., et al.
  2. Early neutralizing IgG response to Chikungunya virus in infected patients targets a dominant linear epitope on the E2 glycoprotein. EMBO Mol Med. (2012) 4 (4): 330-43. Kam Y.W., et al.
  3. Enhanced production of Chikungunya virus-like particles using a high-pH adapted spodoptera frugiperda insect cell line. PLoS One. (2014) 9 (4): e94401. Wagner J.M., et al.
  4. Risk factors for symptomatic and asymptomatic chikungunya infection. Trans R Soc Trop Med Hyg. (2013) 107 (12): 789-96. Nakkhara P., et al.
  5. Inflammatory cytokine expression is associated with chikungunya virus resolution and symptom severity. PLoS Negl Trop Dis. (2011) 5 (8): e1279. Kelvin A.A., et al.

ADDITIONAL INFORMATION

  • Full Name: Chikungunya Virus IgG Capture ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: diagn. >90%

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