Human CD105 (endogulin) ELISA kit is a reliable quantitative procedure for measuring endoglin (CD105) in human serum, cell culture supernatant, plasma, body fluids and tissue lysates. This assay has a minimum sensitivity detection limit of 0.094 ng/ml.
CD105 (endogulin) is a homo-dimeric glycoprotein that is 180kDA, it is associated primarily with human vascular endothelium. It contains disulfide links at cysteines 350 and 582 in order to form the homodimers, it also has a large 561 amino acids extracellular domain, a short 45 amino acids cytoplasmic tail domain and a hydrophobic transmembrane domain. The endoglin (CD105) gene can be found located on human chromosome 9 with location of the cytogenic band at 9q34.1, the coding region of the gene is made up of 14 exons.
CD105 has important roles in angiogenesis, it is a vital protein for tumor growth, survival and metastasis of many different cancer cells to other locations within the body. Endoglin has been implicated in roles relating to the development of the cardiovascular system and in vascular remodelling, its expression is regulated during heart development. It has been further postulated that CD105 (endoglin) could be is involved in the cytoskeletal organisation which is affecting cell migration and morphology.
The standard used in the following endogulin/CD105 ELISA kit is an extracellular part of recombinant human endogulin (CD105), from E26 to G586 and as a result of glycosylation, the total molecular mass is 75-85KDa. Endoglin is predominately found in auxiliary receptor for the TGF-beta receptor complex and can function in modulating a response following the binding of activin-A, TGF-beta1, TGF-beta3, BMP-2 and BMP-7. In humans endoglin has been found to be linked to autosomal dominant disorders that are also known as hereditary hemorrhagic telangiectasia (HHT) type. Also the expression of CD105 and TGF-beta secretion are attenuated in bone marrow stromal cells when they are co-cultured alongside prostate cancer cells.
CD105 (ENDOGULIN) ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Standards: Concentrations 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5.0, 10.0 ng/ml, made up from lyophilized recombinant human endogulin (CD105).
- One 96-Well Plate: Pre-coated with anti CD105/Endogulin antibody.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled CD105 (Endogulin) Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
The minimum sensitivity detection limit of CD105 (endoglin) using current human endoglin/CD105 ELISA kit was approximately 0.094 ng/ml. The dynamic range for this assay is 0.156 – 10.0 ng/ml.
– CD105 (Endoglin): ELISA
– Specificity: Highly specific for detecting endoglin (ENG), no cross reactivity or interference between other endoglin/CD105 analogues was detected.
– Recovery: Serum (90 – 100%), EDTA Plasma (85 – 103%), Heparin Plasma (91 – 102%).
– Linearity: Serum (85 – 104%), EDTA Plasma (83 – 100%), Heparin Plasma (84 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10% within the expiration date.
- Endoglin (CD105) expression and angiogenesis status in small cell lung cancer. Pathol Res Pract. (2010) 206 (11): 725-30. Takase Y., et al.
- CD105 is a marker of tumour vasculature and a potential target for the treatment of head and neck squamous cell carcinoma. J Oral Pathol Med. (2010) 39 (5): 361-7. Review. Marioni G., et al.
- Assessment of angiogenesis by CD105 and nestin expression in peritumor tissue of glioblastoma. Int J Oncol. (2011) 38 (1): 41-9. Sica G., et al.
- Full Name: CD105 (Endoglin) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma, Tissue Lysate, Body Fluids, Cell Culture Supernatant
- Sensitivity: 0.094 ng/ml