Beta-Defensin 1 ELISA Kit (DEFB1)

INTENDED USE

Human beta-defensin 1 ELISA kit can detect amounts of DEFB1 (beta-defensin 1, BD-1, HBD1) present is human biological fluids, serum, plasma and tissue homogenate samples.

INTRODUCTION

Beta-defensin 1 (DEFB1) is a member of the defensin family which are small proteins (15-20 residues) that function in antimicrobial defense by penetrating a microbe’s cell membrane and cause microbial death in a manner similar to that of antibiotics. It is an antibacterial peptide that epithelial cells on mucosal surfaces constitutively express. Beta-defensins can be found in various forms in the nose passage, lungs, saliva, and breast milk. Humans express three different types of beta-defensins, which all have antimicrobial properties. The expression of DEFB1 is regulated by a number of environmental conditions including the air in which an individual’s members are raised. For example, the secretion of defensins in newborn is high during respiratory infections but drops off as the infant gains immunity.

Beta-defensin 1 is also detected, though at considerably lower levels, in people with cystic fibrosis or asbestos exposure. Breastfeeding can boost beta-defensin levels, and low levels have been linked to greater illness vulnerability as well as higher risks of asthma and allergy-related death. It is present in relatively small amounts in perspiration, urine and saliva. Formation of beta-defensins occurs through a process called protein processing.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-DEFB1 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this DEFB1 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for DEFB1, no cross reactivity or interference between DEFB1 and analogues was detected.
– Recovery: Serum (89 – 103%), EDTA Plasma (86 – 102%), Heparin Plasma (85 – 103%).
– Linearity: Serum (88 – 103%), EDTA Plasma (84 – 98%), Heparin Plasma (81 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Modulation of Human β-Defensin-1 Production by Viruses. (2017) 9 (6): 153. Ryan L.K. and Diamond G.
2. Association between DEFB1polymorphisms and periodontitis: a meta-analysis. (2019) 74 (7): 390-396. Zhong S., et al.
3. Variation in human β-defensin genes: new insights from a multi-population study. Int J Immunogenet. (2013) 40 (4): 261-9. Mehlotra R.K., et al.
4. Human-beta-defensin 1: a restless warrior against allergies, infections and cancer. Int J Biochem Cell Biol. (2010) 42 (6): 800-4. Prado-Montes de Oca E.

ADDITIONAL INFORMATION

• Full Name: Beta-Defensin 1 ELISA Kit (DEFB1)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 18.75 pg/ml

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