ACE2 ELISA Kit (Angiotensin-Converting Enzyme 2)

Full Name: ACE2 ELISA Kit (Angiotensin-Converting Enzyme 2)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.234 ng/ml

INTRODUCTION

Angiotensin-converting enzyme 2 (ACE2) exists either in the soluble form (sACE2) or attached to the membrane of cells (mACE2). sACE2 is a soluble protein which acts as an enzyme in the body. It converts angiotensin I to angiotensin II. Two kinase reactions then take place on either side of ACE2 where it is catalyzed by two different enzymes, converting angiotensin II to Ang-(1-7) and then to Ang-(1-9). The latter reaction results in aldosterone synthesis. Ang-(1-7) and Ang-(1-9) bind to the receptor cells causing an increase in volume of blood plasma, which increases cardiac output and hydrostatic pressure (baroreceptor reflex). Ang-(1-9) is then converted to aldosterone which increases renal reabsorption of sodium, chloride and water. It also inhibits the release of renin from α cells of the juxtaglomerular apparatus and increases activity of NaCl cotransport.

Both membrane-bound and soluble forms are integral parts of the renin–angiotensin–aldosterone system (RAAS) that exists to keep the body’s blood pressure in check. It has an important function as part of the renin–angiotensin–aldosterone system (RAAS) protective phase, where soluble ACE2’s (sACE2) act as a counterbalance to the angiotensin-converting enzyme (ACE). sACE2 lowers blood pressure by catalyzing the hydrolysis of angiotensin II (a vasoconstrictor peptide) into angiotensin (1–7)(a vasodilator) which in turns binds to MasR receptors creating localized vasodilation and hence decreasing blood pressure.

It also acts as a vasodilator, which counterbalances ACE and causes blood vessel walls to relax. Due to its important role, ACE2 is also highly regulated by the renin–angiotensin system (RAS) which is a part of the body’s intrinsic metabolic pathway. According to this regulation, when blood pressure rises and becomes too high, the RAS induces activation of ACE to produce more angiotensins which causes vasoconstriction and raises the blood pressure. It is inhibited by angiotensin II (AngII), renin and post-translational modifications. It is referred to as as a regulator of extracellular matrix protein degradation.

INTENDED USE

Human ACE2 ELISA kit can be used to analyse levels of angiotensin-converting enzyme 2 (ACE2, ACEH, ACEII) present is serum, plasma, biological fluids and tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti-ACE2 antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this ACE2 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.391, 0.781, 1.562, 3.125, 6.25, 12.5, 25 ng/ml.
Reactivity: Human
Sensitivity: 0.234 ng/ml
Range: 0.391 – 25 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for ACE2, no cross reactivity or interference between ACE2 and analogues was detected.
– Recovery: Serum (86 – 105%), EDTA Plasma (85 – 99%), Heparin Plasma (86 – 99%).
– Linearity: Serum (89 – 98%), EDTA Plasma (81 – 100%), Heparin Plasma (90 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

ACE2_pathway regulates thermogenesis and energy metabolism. (2022) 11: e72266. Cao X., et al.
Angiotensin-converting enzyme 2 (ACE2): SARS-CoV-2 receptor and RAS modulator. Acta Pharm Sin B. (2021) 11 (1): 1-12. Bian J. and Li Z.
ACE2, angiotensin 1-7 and skeletal muscle: review in the era of COVID-19. Clin Sci (Lond). (2020) 134 (22): 3047-3062. Yamamoto K., et al.
Soluble-ACE2 Is Filtered into the Urine. m (2022) 3 (12): 2086-2094. Nelson J.W., et al.

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