GET A QUOTE

Antigen KI-67 ELISA Kit (MKI67)

Full Name: Antigen KI-67 ELISA Kit (MKI67)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.188 ng/ml

INTRODUCTION

Antigen KI-67 represents a nuclear protein universally expressed among actively proliferating cells, but nearly undetectable in quiescent cells. Its identification emerged in 1983 when researchers derived a monoclonal antibody from mice immunized with Hodgkin lymphoma nuclei. This antibody detected a nuclear target antigen in proliferating cells, hence termed Ki-67 after the researchers’ city of origin. Despite ubiquitous use today as a clinical proliferation marker, the Ki-67 protein itself took over 20 additional years to isolate and characterize.

Molecular cloning reveals a large multi-domain protein containing 16 concatenated “Ki-67 repeats” split by linker regions. Within the nucleus, Ki-67 dynamically associates with condensed chromosomes during mitosis, but relocates to the nucleolus periphery in interphase – mediating ribosome RNA synthesis critical for proliferation. Still the necessity of Ki-67 for cell cycle progression went unproven until genetic knockout approaches became possible in the 2000s. Strikingly Ki-67 null mice display severe atrophy in self-renewing organs caused by impaired progenitor cell division.

Today immunohistochemistry using Ki-67 antibodies to score proliferative activity sees ubiquitous use in cancer pathology. However, emerging strategies blocking Ki-67 transcription or splicing show early promise in reducing proliferation of tumor cells and self-renewing stem cells without toxicity. As the crucial role of Ki-67 enabling cell proliferation becomes ever clearer, therapeutic targeting of this multifaceted nuclear protein continues gaining increased attention.

INTENDED USE

Human antigen KI-67 ELISA kit can measure concentrations of MKI67 (antigen KI-67, Ki-67, TSG126, KIA) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti- MKI67.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this antigen KI-67 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for MKI67, no cross reactivity or interference between MKI67 and analogues was detected.
– Recovery: Serum (88 – 105%), EDTA Plasma (86 – 102%), Heparin Plasma (86 – 96%).
– Linearity: Serum (87 – 92%), EDTA Plasma (84 – 95%), Heparin Plasma (81 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. The cell proliferation antigen Ki-67 organises heterochromatin. Elife. (2016) 5: e13722. Sobecki M., et al.
  2. The Biomarker Ki-67: Promise, Potential, and Problems in Breast Cancer. Appl Immunohistochem Mol Morphol. (2023) 31 (7): 478-484. Gown AM.
  3. The Ki-67 antigen in the new 2021 World Health Organization classification of lung neuroendocrine neoplasms. Pathologica. (2021) 113 (5): 377-387. Pelosi G. and Travis W.D.
  4. Ki-67 regulates global gene expression and promotes sequential stages of carcinogenesis. Proc Natl Acad Sci U S A. (2021) 118 (10): e2026507118. Mrouj K., et al.

OTHER RELATED ELISA KITS

Online Enquiry Form

"*" indicates required fields

Please check mark information required:

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford