Antigen KI-67 ELISA Kit (MKI67)

Full Name: Antigen KI-67 ELISA Kit (MKI67)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.188 ng/ml


Antigen KI-67 represents a nuclear protein universally expressed among actively proliferating cells, but nearly undetectable in quiescent cells. Its identification emerged in 1983 when researchers derived a monoclonal antibody from mice immunized with Hodgkin lymphoma nuclei. This antibody detected a nuclear target antigen in proliferating cells, hence termed Ki-67 after the researchers’ city of origin. Despite ubiquitous use today as a clinical proliferation marker, the Ki-67 protein itself took over 20 additional years to isolate and characterize.

Molecular cloning reveals a large multi-domain protein containing 16 concatenated “Ki-67 repeats” split by linker regions. Within the nucleus, Ki-67 dynamically associates with condensed chromosomes during mitosis, but relocates to the nucleolus periphery in interphase – mediating ribosome RNA synthesis critical for proliferation. Still the necessity of Ki-67 for cell cycle progression went unproven until genetic knockout approaches became possible in the 2000s. Strikingly Ki-67 null mice display severe atrophy in self-renewing organs caused by impaired progenitor cell division.

Today immunohistochemistry using Ki-67 antibodies to score proliferative activity sees ubiquitous use in cancer pathology. However, emerging strategies blocking Ki-67 transcription or splicing show early promise in reducing proliferation of tumor cells and self-renewing stem cells without toxicity. As the crucial role of Ki-67 enabling cell proliferation becomes ever clearer, therapeutic targeting of this multifaceted nuclear protein continues gaining increased attention.


Human antigen KI-67 ELISA kit can measure concentrations of MKI67 (antigen KI-67, Ki-67, TSG126, KIA) present is plasma, serum, biological fluids or tissue homogenate samples.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti- MKI67.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.


For this antigen KI-67 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.


– Specificity: Highly specific for MKI67, no cross reactivity or interference between MKI67 and analogues was detected.
– Recovery: Serum (88 – 105%), EDTA Plasma (86 – 102%), Heparin Plasma (86 – 96%).
– Linearity: Serum (87 – 92%), EDTA Plasma (84 – 95%), Heparin Plasma (81 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%


  1. The cell proliferation antigen Ki-67 organises heterochromatin. Elife. (2016) 5: e13722. Sobecki M., et al.
  2. The Biomarker Ki-67: Promise, Potential, and Problems in Breast Cancer. Appl Immunohistochem Mol Morphol. (2023) 31 (7): 478-484. Gown AM.
  3. The Ki-67 antigen in the new 2021 World Health Organization classification of lung neuroendocrine neoplasms. Pathologica. (2021) 113 (5): 377-387. Pelosi G. and Travis W.D.
  4. Ki-67 regulates global gene expression and promotes sequential stages of carcinogenesis. Proc Natl Acad Sci U S A. (2021) 118 (10): e2026507118. Mrouj K., et al.


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