Annexin V ELISA Kit (ANX-V)

INTENDED USE

Human annexin V ELISA kit can be used for analysing in vitro quantitative levels of annexin V (ANX-V, annexin A5) in human biological fluids, plasma, serum and tissue homogenates. This assay has a minimum sensitivity limit of 0.938 ng/ml.

BACKGROUND

Annexin V which is also usually called annexin A5 is a cellular protein which has been useful in detecting apoptotic cells due to their ability to bind phosphatidylserine (a common marker for apoptosis). There are many publication indicating that it plays a vital in inhibiting blood coagulation through competing with prothrombin for phosphatidylserine binding sites and it is able to inhibit the activity of phospholipase A1.

Annexin V is often used as a non-quantitative probe in order to detect cells which are expressing phosphatidylserine on the cell surface, this event is common is cells that are undergoing death or apoptosis. It has been shown to interact with Integrin, beta 5 and kinase insert domain receptor and also to be upregulated in papillary thyroid carcinoma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti ANX-A5 antibody.
• Standards: Concentrations 0, 0.1562, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of annexin V (annexin-A5, ANX-V) using this ANX-V ELISA kit was 0.938 ng/ml. The dynamic range for this assay is 1.563 – 100.0 ng/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for ANX-A5, no cross reactivity or interference between ANX-A5 and analogues was detected.
– Recovery: Serum (88 – 102%), EDTA Plasma (90 – 102%), Heparin Plasma (85 – 103%).
– Linearity: Serum (90 – 101%), EDTA Plasma (82 – 95%), Heparin Plasma (81 – 93%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Annexin A5 as a potential marker in tumors. Clin Chim Acta. (2014) 427: 42-8. Review. Peng B., et al.
2. Annexin V, the regulator of phosphatidylserine-catalyzed inflammation and coagulation during apoptosis. Cell Mol Life Sci. (1997) 53 (6): 527-32. Review. Reutelingsperger C.P. and van Heerde W.L.
3. Anti-annexins autoantibodies: their role as biomarkers of autoimmune diseases. Autoimmun Rev. (2011) 10 (9): 553-8. Review. Iaccarino L., et al.
4. Apoptosis imaging: beyond annexin V. J Nucl Med. (2010) 51 (11): 1659-62. Review. Niu G. and Chen X.

ADDITIONAL INFORMATION

• Full Name: Annexin V ELISA Kit (ANX-V)
• Reactivity: Human
• Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
• Sensitivity: 0.938 ng/ml

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