Angiotensin 1-7 ELISA Kit (Ang1-7)

INTENDED USE

Human angiotensin 1-7 ELISA kit can measure concentrations of Ang1-7 (angiotensin 1-7) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

Angiotensin 1-7 (Ang1-7) is a hormone, in the renin angiotensin system (RAS) that works as a vasodilator counteracting the effects of angiotensin II (AngII). It is produced mainly from AngII by an enzyme called angiotensin converting enzyme 2 (ACE2). When it binds to the receptor (MasR) Ang1-7 has effects on the heart, inflammation, fibrosis and even tumor growth by dampening AngII mediated signaling.

While traditionally seen as a hormone that circulates throughout the body Ang1-7 likely also acts as a mediator within tissues influencing responses through RAS enzymes found in organs. By inhibiting pathways that promote growth Ang1-7 regulates processes like blood pressure regulation, glucose and lipid metabolism. Tissue remodeling. This is particularly relevant in organs such as the pancreas, heart, kidney and central nervous system. In animal studies administering Ang1-7 has shown results in preventing heart failure progression. It has also been effective in reducing damage, to the kidneys after injury by improving blood flow and filtration while suppressing responses and preventing excessive deposition of extracellular matrix through TGF beta inhibition.

Considering the effects of the ACE2 Ang1-7 pathway, against dysfunction, atherogenesis, arrhythmia and hypertension typically induced by the ACE AngII pathway there has been growing interest in selectively activating Ang1-7 signaling as a promising therapeutic approach, for cardiometabolic diseases. Furthermore investigating the mechanisms underlying tissue specific 7 bioactivity could reveal new targets along its signaling cascade that can be manipulated more precisely to achieve downstream antifibrotic, vasculoprotective and cytostatic effects.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-Ang1-7 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this Ang1-7 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for Ang1-7, no cross reactivity or interference between Ang1-7 and analogues was detected.
– Recovery: Serum (87 – 103%), EDTA Plasma (89 – 104%), Heparin Plasma (91 – 99%).
– Linearity: Serum (85 – 100%), EDTA Plasma (82 – 101%), Heparin Plasma (86 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Angiotensin-(1-7), Adipokines and Inflammation. Metabolism. (2019) 95: 36-45. Lelis D.F., et al.
2. The ACE-2/Ang1-7/Mas cascade enhances bone structure and metabolism following angiotensin-II type 1 receptor blockade. Eur J Pharmacol. (2017) 807: 44-55. Abuohashish H.M., et al.
3. The renin-angiotensin system. Contrib Nephrol. (2011) 170: 135-144. Lai K.N., et al.
4. Improved pharmacokinetics and bone tissue accumulation of Angiotensin-(1-7) peptide through bisphosphonate conjugation. Amino Acids. (2021) 53 (5): 653-664. Aghazadeh-Habashi A. and Khajehpour S.

ADDITIONAL INFORMATION

• Full Name: Angiotensin 1-7 ELISA Kit (Ang1-7)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 9.375 ng/ml

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