Gliadin IgM ELISA Kit

INTENDED USE

Human gliadin IgM ELISA kit can be used for detecting in-vitro quantitative concentrations of human IgM antibodies against gliadin (gliadin IgM) in plasma or serum. This assay has a minimum analytical sensitivity limit of 0.94 U/ml.

BACKGROUND

Three subtypes of anti-gliadin antibodies (AGA) exist: anti-gliadin IgA, anti-gliadin IgG, and anti-gliadin IgE. They are found in wheat as prolamin, and are formed in reaction to gliadin. They have three alleles: AA, BB, or DD. Due to the small differences between each allele, several antibodies can be produced. They are becoming increasingly frequently used in the diagnosis of gluten-induced enteropathy and make up essential parts of gluten.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Strips: 12 strips with 8 breakable wells each, coated with a gliadin antigen (purified gluten antigen from wheat).
• Calibrator A (Negative Control): No IgM antibodies against gliadin.
• Calibrator B (Cut-Off Standard): Low concentration of IgM antibodies against gliadin.
• Calibrator C (Weak Positive Control): Medium concentration of IgM antibodies against gliadin.
• Calibrator D (Positive Control): High concentration of IgM antibodies against gliadin.
• Enzyme Conjugate: Containing anti-human-IgM-HRP (rabbit), in protein-containing buffer solution.
• Substrate Solution: Tetramethylbenzidine (TMB).
• Stop Solution: 1 N acidic solution.
• Sample Diluent.
• Washing Buffer (10x).

SENSITIVITY

The minimum detection sensitivity level of human anti-gliadin IgM (gliadin-IgM) using current gliadin IgM ELISA kit was 0.94 U/ml. The dynamic range for this assay is 1.0 – 300.0 U/ml.

ASSAY CHARACTERISTICS

– Intra Assay Precision: 8.6%
– Inter Assay Precision: 9.8%
– Inter-Lot Precision: 4.0 – 13.1 %
– Analytical Sensitivity: 0.94 U/mL
– Recovery: 72 – 106 %
– Linearity: 79 – 123 %
– Cross-Reactivity: No cross-reactivity to TPO, Transglutaminase, dsDNA  and TG.
– Interferences: None

REFERENCES

1. IgG, IgA and IgE gliadin antibody determinations as screening test for untreated coeliac disease in children, a multicentre study. Eur J Pediatr. (1989) 148 (6): 496-502. Bürgin-Wolff A., et al.
2. Class and subclass-associated specificity differences of anti-gliadin antibodies from mucosa and serum. Immunology. (1992) 77 (4): 604-8. Engström P.E., et al.
3. Secretion of gliadin antibody by coeliac jejunal mucosal biopsies cultured in vitro. Clin Exp Immunol. (1986) 64 (1): 119-24. Ciclitira P.J., et al.
4. Gliadin antibody production by small intestinal lymphocytes from patients with coeliac disease. Int Arch Allergy Appl Immunol. (1989) 89 (2-3): 246-9. Ciclitira P.J., et al.
5. Immunoreactivity to food antigens in patients with chronic urticaria. Immunol Invest. (2014) 43 (5): 504-16. Rašković S., et al.

ADDITIONAL INFORMATION

• Full Name: Gliadin IgM ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity: 0.94 U/ml

OTHER RELATED ELISA KITS

• Gliadin IgG ELISA Kit
• Gliadin IgA ELISA Kit
• GBM ELISA Kit