Endostatin ELISA kit

INTENDED USE

Human endostatin ELISA kit can detect amounts of endostatin (Es, Estrogen) present is human plasma, serum, biological fluids and tissue homogenates.

INTRODUCTION

Even though attention in vascular science often goes to substances driving blood vessel development, the body also relies on natural shutdown mechanisms to limit such growth. One strong example is Endostatin, a small protein piece – about 20 kilodaltons in size – that forms when collagen XVIII, part of vessel walls, breaks down. First identified toward the end of the 1990s, it works by slowing down the creation of fresh capillaries. What stands out is how it locks onto endothelial cells, blocking vital growth prompts. Instead of supporting vessel formation, it hinders movement of cells toward growing sites. Over time, these same cells face removal through apoptosis, a natural shutdown mechanism. Because of this, steady suppression of angiogenesis becomes possible.

What makes endostatin useful is how it targets tumor growth. Since cancer keeps making new blood vessels, blocking that process offers strong potential. Instead of creating a new drug, scientists used a version built from human genes. This form of endostatin entered clinical trials, first seen in widespread use across Chinese medical settings. Starving tumors of blood means less room for growth, slowing spread. It blocks key routes cancer uses to get nutrients. Multiple actions unfold at once, hitting different signals in the body. Chasing one path only can lead to resistance; this approach splits attention between targets. Simultaneous pressure may keep diseases from adapting too easily.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-endostatin antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Stop Solution
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this endostatin ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5.0, 10.0 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for ES, no cross reactivity or interference between ES and analogues was detected.
– Recovery: Serum (85 – 101%), EDTA Plasma (88 – 100%), Heparin Plasma (88 – 105%).
– Linearity: Serum (86 – 99%), EDTA Plasma (86 – 99%), Heparin Plasma (85 – 100%).
– Precison Intra-Assay: CV < 5%.
– Precison Inter-Assay: CV < 5%.

REFERENCES

1. Endostatin: a promising biomarker in the cardiovascular continuum? Biomark Med. (2017) 11(10) :905-916. Ruge T., et al.
2. Endostatin-based anti-angiogenic therapy and immune modulation: mechanisms and synergistic potential in cancer treatment. Front Immunol. (2025) 16 :1623859. Sun J., et al.
3. Endostatin’s emerging roles in angiogenesis, lymphangiogenesis, disease, and clinical applications. mBiochim Biophys Acta. (2015) 1850 (12):2422-38. Walia A., et al.
4. Endostatin: current concepts about its biological role and mechanisms of action. Biochemistry (Mosc). (2007)72 (3):235-46. Digtyar A.V., et al.

ADDITIONAL INFORMATION

• Full Name: Endostatin ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids
• Sensitivity: 0.094 ng/ml

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