Sirtuin 1 ELISA Kit (SIRT1)

INTENDED USE

Human sirtuin 1 ELISA kit can measure concentrations of SIRT1 (sirtuin 1, SIR2-like protein 1, homolg 1, EC 3.5.1, SIR2alpha) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 0.188 ng/ml.

INTRODUCTION

SIRT1, known as sirtuin 1, is a NAD-dependent deacetylase, it removes acetyl groups from target proteins to modulate their activation states in response to metabolic or environmental signals. The longevity and health-related effects of calorie restriction relate partly to SIRT1 activity enhancement.

SIRT1 deacetylates histones in chromosomal DNA as well as various transcription factors and co-regulators to influence gene expression patterns. This alters balances between anabolism and catabolism, oxidative stress responses, apoptosis signaling, and circadian behaviors in cells. For instance, SIRT-1 enhances fat mobilization, inhibits fat storage/growth pathways, stimulates mitochondrial biogenesis, reduces inflammation through transcription factor regulation, and beneficially impacts circadian clock maintenance – effects amplified by nutrient deprivation.

Such SIRT1 regulatory impacts likely contribute to improved health and survival observed with calorie restriction in some species. Boosting its activity pharmacologically aims to mimic such effects. Compound screening reveals small molecule sirtuin activating compounds (STACs) can enhance SIRT1 activity to realize therapeutic effects in metabolic, neurodegenerative, inflammatory, and aging-related disease models. However, designing isoform-selective SIRT-1 modulators has proved challenging. Off-target effects and incomplete understanding of downstream mechanisms still require further study.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-Sirtuin 1.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this sirtuin 1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml.
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for SIRT1, no cross reactivity or interference between SIRT1 and analogues was detected.
– Recovery: Serum (86 – 105%), EDTA Plasma (90 – 104%), Heparin Plasma (86 – 104%).
– Linearity: Serum (86 – 98%), EDTA Plasma (82 – 100%), Heparin Plasma (82 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. SIRT1 and aging related signaling pathways. Mech Ageing Dev. (2020) 187: 111215. Chen C., et al.
2. Sirtuin 1 (SIRT1) and steroid hormone receptor activity in cancer. J Endocrinol. (2012) 213 (1): 37-48. Moore R.L., et al.
3. The Beneficial Roles of SIRT1 in Neuroinflammation-Related Diseases. Oxid Med Cell Longev. (2020) 2020: 6782872. Jiao F. and Gong Z.
4. Sirtuin 1 and Alzheimer’s disease: An up-to-date review. Neuropeptides. (2018) 71: 54-60. Rizzi L. and Roriz-Cruz M.

ADDITIONAL INFORMATION

• Full Name: Sirtuin 1 ELISA Kit (SIRT1)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.188 ng/ml

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