Pleiotrophin ELISA Kit (PTN)

INTENDED USE

Human pleiotrophin ELISA kit can measure concentrations of PTN (pleiotrophin, OSF-1, HBNF-1, HB-GAM, HBBM, HBGF-8) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 18.75 ng/ml.

INTRODUCTION

PTN, or pleiotrophin, is a developmentally regulated protein that is highly concentrated in the brain and bone during neonatal development but expressed at lower levels in many tissues in adults. By binding cell surface receptors, pleiotrophin activates signaling influencing cell proliferation, migration, and survival.

Expressed early in development, it is critical for normal brain, bone, and epithelial cell growth. Through boosted protein and DNA synthesis, it enhances cell division rates and tissue expansion. It also participates in synapse formation and neural process growth. Following maturation, continued PTN expression facilitates wound healing responses in bone fractures and vascularization. However, overactive or prolonged PTN signaling is associated with pathological excess cell proliferation in some cancers and inflammatory disease progression by exacerbating abnormal angiogenesis and cell migration.

Pleiotrophin’s receptor binding activates major cellular pathways like MAPK and PI3K/AKT. This alters gene transcription to induce proteins enabling cell cycle progression and inhibiting apoptosis. PTN additionally interacts with extracellular matrix proteins and cytokines to stimulate changes benefitting cell migration, adhesion, and intercellular communication.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- PTN.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this pleiotrophin ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PTN, no cross reactivity or interference between PTN and analogues was detected.
– Recovery: Serum (91 – 101%), EDTA Plasma (86 – 97%), Heparin Plasma (86 – 103%).
– Linearity: Serum (85 – 98%), EDTA Plasma (90 – 101%), Heparin Plasma (82 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Pleiotrophin: Activity and mechanism. Adv Clin Chem. (2020) 98: 51-89. Wang X.
2. CREB3L1 and PTN expressions correlate with prognosis of brain glioma patients. Biosci Rep. (2018) 38 (3): BSR20170100. Liu L.Q., et al.
3. The role of pleiotrophin in bone repair.Injury. (2014) 45 (12): 1816-23. Lamprou M., et al.

ADDITIONAL INFORMATION

• Full Name: Pleiotrophin ELISA Kit (PTN)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 18.75 pg/ml

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