Cholecystokinin ELISA Kit (CCK)

INTENDED USE

Human cholecystokinin ELISA kit can measure concentrations of CCK (cholecystokinin) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 9.375 pg/ml.

INTRODUCTION

Cholecystokinin, abbreviated CCK, is secreted by I-cells in the mucosal epithelium of the duodenum and jejunum when fats and proteins are detected, it stimulates the release of digestive enzymes and bile, allowing for the breakdown and absorption of food.

By binding CCK-receptors on the pancreatic acinar cells after being released into circulation, it triggers the discharge of pancreatic enzymes into the small intestine. These include proteases, lipases, and amylases which help digest proteins, fats, and carbohydrates from chyme entering the duodenum from the stomach. Additionally, CCK contracts the gallbladder to release stored bile, aiding the digestion and uptake of fat-soluble nutrients. It also has effects on gastric motility and emptying rates based on the volume and composition of intestinally arriving nutrients, thus coordinating stomach and intestinal activity. Furthermore, acting as a hunger suppressant, CCK induces satiety signals communicating fullness via vagal afferent nerves projecting from the gut to feeding centers in the brain.

In addition to facilitating digestion through coordinated regulation of pancreatic, biliary, and gastric functions after nutrient sensing, CCK also appears to mediate analgesic effects. Overall however, the integral role of cholecystokinin in postprandial digestive regulation is well established, from triggering pancreas and gallbladder release to inducing satiation following a meal.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- CCK.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this CCK ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CCK, no cross reactivity or interference between CCK and analogues was detected.
– Recovery: Serum (91 – 105%), EDTA Plasma (88 – 102%), Heparin Plasma (87 – 99%).
– Linearity: Serum (89 – 104%), EDTA Plasma (89 – 99%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Cholecystokinin in the central nervous system: a minireview. Neuropeptides. (1983) 3 (6): 411-27. Beinfeld M.C.
2. Effects of CCK on pancreatic function and morphology. Ann N Y Acad Sci. (1994) 713: 180-98. Niederau C., et al.
3. [Cholecystokinin (CCK). Physiology and clinical use]. Klin Wochenschr. (1970) 48 (2): 65-71. Jorpes E. and Mutt V.
4. Bioassay of cholecystokinin. Gut. (1982) 23 (2): 146-9. Giannoulis E.L. and Barry R.E.

ADDITIONAL INFORMATION

• Full Name: Cholecystokinin ELISA Kit (CCK)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 9.375 pg/ml

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