ICTP ELISA Kit

INTENDED USE

Human ICTP ELISA kit can measure concentrations of cross-linked C-terminal telopeptide of collagen alpha-1 (ICTP) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

ICTP, or cross-linked C-telopeptide of type I collagen, constitutes a peptide degradation product generated by osteoclastic resorption of mature, crosslinked collagen type I fibrils that make up the bone matrix. As bone tissue undergoes constant turnover mediated by opposing activity of osteoblasts and osteoclasts, biomarkers of the coordinated remodeling process provide critical insight into prevailing bone balance in research and clinical contexts. In particular, immunoassays detecting ICTP in serum or urine offer specific quantification of catabolic activity.

During the breakdown phase of bone remodeling handled by osteoclasts, proteolytic enzymes like cathepsin K hydrolyze the crosslinked collagen meshwork constituting the mineralized matrix. This liberates small peptide fragments from type I collagen in bone including ICTP, which then enter circulation for renal clearance. Since generation of this C-terminal telopeptide requires prior formation and maturation of crosslink-stabilized collagen before subsequent osteoclastic digestion, ICTP levels closely reflect bone resorption activity.

Correspondingly, elevated ICTP strongly associates with high-turnover osteoporosis, correlating with risk factors like postmenopausal status. ICTP also augments in conditions like hyperparathyroidism, Paget’s disease, and bone metastases exhibiting uncontrolled osteoclastic activity. As such, quantitative ICTP enzyme immunoassays facilitate disease screening, treatment monitoring to slow excess resorption, and fracture risk prognostication. Similar assays also show promise in assessing cartilage catabolism and rheumatoid arthritis disease activity representing other areas of collagen degradation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti-ICTP antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this ICTP ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 78.125, 156.25, 312.5, 625, 1250, 5000, 5000 pg/ml.
Reactivity: Human
Sensitivity: 46.875 pg/ml
Range: 78.125 – 5000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for ICTP, no cross reactivity or interference between ICTP and analogues was detected.
– Recovery: Serum (85 – 105%), EDTA Plasma (91 – 102%), Heparin Plasma (85 – 102%).
– Linearity: Serum (85 – 100%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

Polymeric Materials: Surfaces, Interfaces and Bioapplications. Materials (Basel). (2019) 12 (8): 1312. Muñoz-Bonilla A., et al.
Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area. Dent Mater. (2018) 34 (3): 452-459. Turco G., et al.
Carboxyterminal telopeptide of type I collagen (ICTP) in predicting prognosis in epithelial ovarian cancer. Gynecol Oncol. (2001) 82 (1): 110-5. Simojoki M., et al.
The type I collagen fragments ICTP and CTX reveal distinct enzymatic pathways of bone collagen degradation. J Bone Miner Res. (2003) 18 (5): 859-67. Garnero P., et al.

ADDITIONAL INFORMATION

Full Name: ICTP ELISA Kit (Cross-Linked C-Terminal Telopeptide of Collagen Alpha-1(I) Chain)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 46.875 pg/ml

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