IL-33 ELISA Kit (Interleukin-33)

INTENDED USE

Human IL-33 ELISA kit can measure concentrations of interleukin-33 (IL-33) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum detection limit of 9.375 pg/ml.

INTRODUCTION

Interleukin 33 (IL-33) is a molecule that acts as a warning signal, for tissue damage and alerts the system. It achieves this by binding to its receptor ST2 (IL1RL1) and the co receptor IL 1 receptor accessory protein (IL 1RAcP) which activates downstream signaling pathways like factor κB (NF κB). Mitogen activated protein kinase (MAPK).

When cells in our body are injured IL-33 is released passively from the nucleus of barrier cells such as epithelial cells. In cells it is anchored within chromatin by its C chromatin binding motif. However when cell death occurs due to necrosis it quickly diffuses out of the chromatin. Additionally under conditions bioactive full length IL-33 is actively secreted. Once released it affects types of cells including group 2 innate lymphoid cells T helper 2 lymphocytes, regulatory T cells, basophils, eosinophils, mast cells, macrophages, dendritic cells and natural killer cells. As a result of these interactions with cells IL-33 plays roles in type 2 inflammation that contributes to allergic diseases like asthma, atopic dermatitis and anaphylaxis.

However it’s important to note that IL-33 doesn’t solely promote inflammation. Its influence on lymphocytes helps suppress T cell responses and maintain tolerance towards harmless substances such, as food antigens or commensal microbes. Furthermore it prompts the release of cancer fighting cytokines, by killer cells and CD8+ T cells. As a result the signaling of IL-33 effectively maintains an equilibrium, between activation and regulation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-IL-33 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this IL-33 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-33, no cross reactivity or interference between IL-33  and analogues was detected.
– Recovery: Serum (95 – 102%), EDTA Plasma (95 – 99%), Heparin Plasma (85 – 98%).
– Linearity: Serum (85 – 98%), EDTA Plasma (90 – 98%), Heparin Plasma (80 – 94%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. IL-23 and axial disease: do they come together? Rheumatology (Oxford). (2021) 60 (Suppl 4): iv28-iv33. Mease P. and van den Bosch F.
2. Interleukin-23: A New Atherosclerosis Target. J Interferon Cytokine Res. (2018) 38 (10): 440-444. Liu W., et al.
3. Anti-IL 23 biologics for the treatment of plaque psoriasis. Expert Opin Biol Ther. (2022) 22 (12): 1489-1502. Vu A., et al.
4. Interleukin 23 and autoimmune diseases: current and possible future therapies. Inflamm Res. (2020) 69 (5): 463-480. Abdo A.I.K. and Tye G.J.

ADDITIONAL INFORMATION

• Full Name: IL-33 ELISA Kit (Interleukin-33)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 9.375 pg/ml

OTHER RELATED ELISA KITS

• Mucin-1 ELISA Kit
• Lipopolysaccharide-Binding Protein ELISA Kit
• NOS2/iNOS ELISA Kit

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