CD24 ELISA Kit (Signal Transducer CD24)

INTENDED USE

Human CD24 ELISA kit can measure concentrations of signal transducer CD24 (CD24, small cell lung carcinoma cluster 4 antigen, CD24A, MGC75943) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 0.094 ng/ml.

INTRODUCTION

CD24 is a heavily glycosylated mucin-like cell surface protein that functions as an adhesive ligand and signaling molecule implicated in a variety of cellular processes. Though anchored to cell membranes by a glycosylphosphatidylinositol linker, it can also exist in soluble form. CD24 is expressed on immature hematopoietic cells but disappears during maturation of most lineages. Consequently it is widely exploited as a phenotypic marker to distinguish lineage-committed hematopoietic progenitors from diverse multipotent progenitor subsets.

The mouse Cd24 gene was originally discovered as a result of the profound lymphocyte deficiencies exhibited in Cd24-null mice. Through src-family kinases and downstream effectors like MAPK, it helps transduce signals from cellular stimuli to elicit functional responses—including proliferation, apoptosis, and altered gene expression—necessary for immune processes. Additionally CD24 can modulate cell adhesion and motility by recruiting cytoskeletal components.

Outside the hematopoietic system, aberrant expression of CD24 has been implicated in progression of diverse carcinoma types as well as neuronal disorders like multiple sclerosis. Malignant potential correlates strongly with deregulated CD24 transcription. Oncogenic signaling pathways converge on the Cd24-promoter, driving overexpression in transformed cells. Cell surface CD24 facilitates interactions within tumor cell niches while secreted CD24 may remodel tissue microenvironments.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-CD24 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this CD24 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CD24, no cross reactivity or interference between CD24 and analogues was detected.
– Recovery: Serum (87 – 99%), EDTA Plasma (86 – 102%), Heparin Plasma (85 – 102%).
– Linearity: Serum (89 – 102%), EDTA Plasma (85 – 99%), Heparin Plasma (80 – 89%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol. (1991) 147 (4): 1412-6. Kay R., et al.
2. CD24 Modulates Chemosensitivity of MCF-7 Breast Cancer Cells. Anticancer Res. (2017) 37 (2): 561-565. Onishi H., et al.
3. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. (1990) 144 (2): 638-41. Fischer G.F., et al.
4. CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas. Cancer Res. (1992) 52 (19): 5264-70. Jackson D., et al.

ADDITIONAL INFORMATION

• Full Name: CD24 ELISA Kit (Signal Transducer CD24)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.094 ng/ml

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