STXBP1 ELISA Kit (Syntaxin-Binding Protein 1)

INTENDED USE

Human STXBP1 ELISA kit can measure concentrations of syntaxin-binding protein 1 (STXBP1, MUNC18, N Sec1, p67, Unc18A) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 0.094 ng/ml.

INTRODUCTION

STXBP1, also referred to as syntaxin binding protein 1 is a component involved in the release of neurotransmitters, from neurons. Its main function is to control the assembly of SNARE complexes which are responsible for vesicle fusion and exocytosis. When STXBP1 malfunctions, it can lead to disorders.

Specifically, STXBP1 interacts with syntaxin proteins located on the neurons target plasma membrane. This interaction allows to regulate the formation of SNARE complexes involving syntaxin, SNAP 25 and synaptobrevin 2. These SNARE proteins work together to bring the vesicle and plasma membrane close facilitating membrane fusion and enabling neurotransmitter release into the synapse.

In the absence of STXBP1 function SNARE complex assembly becomes uncontrolled resulting in impaired neurotransmission. Mutations have been associated with neurodevelopmental conditions. For instance, insufficient levels of STXBP1 can lead to disability epilepsy and features similar to autism. Recent studies have also revealed that certain de novo missense mutations that are linked to epilepsy and encephalopathy.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-STXBP1 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this STXBP1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for STXBP1, no cross reactivity or interference between STXBP1 and analogues was detected.
– Recovery: Serum (90 – 105%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 99%).
– Linearity: Serum (88 – 105%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. STXBP1 encephalopathies: Clinical spectrum, disease mechanisms, and therapeutic strategies. J Neurochem. (2021) 157 (2): 165-178. Abramov D., et al.
2. Essential Role of Syntaxin-Binding Protein-1 in the Regulation of Glucagon-Like Peptide-1 Secretion. Endocrinology. (2020) 161 (5): bqaa039. Campbell J.R., et al.
3. STXBP1 encephalopathies: Clinical spectrum, disease mechanisms, and therapeutic strategies. J Neurochem. (2021) 157 (2): 165-178. Abramov D., et al.
4. A disease concept model for STXBP1-related disorders. Epilepsia Open. (2023) 8 (2): 320-333. Sullivan K.R., et al.

ADDITIONAL INFORMATION

• Full Name: STXBP1 ELISA Kit (Syntaxin-Binding Protein 1)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.094 ng/ml

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• PAR3 ELISA Kit
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