17 Beta Estradiol E2 ELISA Kit


Human 17 beta estradiol E2 ELISA kit is a reliable quantitative procedure for measuring 17-beta estradiol E2 (17β-estradiol E2, 17 beta estradiol E2) in human serum and plasma. This assay has a minimum sensitivity detection limit of 2.0 pg/ml.


17-beta-estradiol (E2) is produced by the placenta and the ovary. It has a molecular weight of 272.4 Da and is a C18 steroid hormone which is in equilibrium with estrone and it can be converted to estriol by the liver or the placenta. It is essentially defined as a steroid hormone which plays important functions in many different types of organs. For, example E2 has a fundamental role in synaptic plasticity, neurogenesis and neuroprotection. There are a number of clinical applications where the determination of E2 levels has been particularly useful. These include the measurement of estradiol levels in peritoneal fluid, serum and follicular fluid as a vital biochemical tool that is routinely used for investigating sexual diseases, tumors, fertility and disorders of hypothalamic/pituitary/gonadal axis.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: 12 x 8 (break apart) strips with 96 wells, coated with 17-beta estradiol E2 antigen.
  • Calibrator 1-5: Concentration 13.0 – 935.0 pg/ml.
  • Control 1 (Low) and Control 2 (High).
  • Enzyme Conjugate: Consisting of antibody labelled horseradish peroxidase in buffered matrix.
  • TMB Substrate Solution.
  • Stop Solution: Contains 2 N hydrochloric acid solution.
  • Wash Solution (10x Concentrated).


The minimum sensitivity detection limit of 17-beta estradiol E2 (17β-estradiol E2) using current 17 beta estradiol E2 ELISA kit was approximately 5.0 pg/ml. The dynamic range for this assay is 13.0 – 935.0 pg/ml.


  1. Subcellular concentrations of estrone, estradiol, androstenedione and 17-β-hydroxysteroid dehydrogenose (17BOH-SOH) Activity in malignant and non-malignant human breast tissues. Int. J. Cancer (1987) 40: 305-308. Metha R.R.
  2. Outcome of in vitro fertilization in women with low response to ovarian stimulation. Fertil. and Steril. (1987) 47 (5): 812-815. Pellicer A., et al.
  3. Dose dependent response of symptoms, pituitary, and bone to transferma oestrogen in postmenopausal women. Br.Med. (1986) 293: 1337-1339. Selby et al.
  4. Ovarian and adrenal contribution to peripheral androgens during the menstrual cycle. J. Clin. Endocrinol. Metab. (1974) 39: 340-346. Abraham G.E.


  • Full Name: 17 Beta Estradiol E2 ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 5.0 pg/ml


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford