Zearalenone ELISA Kit (ZEN)

Full Name: Zearalenone ELISA Kit (ZEN)
Sample Type: Beer/Gyle, Cereal
Sensitivity: 5.0 ppb

BACKGROUND

Zearalenone (ZEN) which is also known by the names of F-2 mycotoxin and RAL is regarded as potent estrogenic metabolite that is formed by some Gibberella and Fusarium species. Particularly examples include: F. graminearum, F. cerealis, F. equiseti, F. culmorum, F. incarnatum and F. verticillioides. These toxins tend to be already produced on the field due to the contact of the cereals by fusarium species. There are a number of Fusarium species which can form toxic substances that can cause considerable concern to poultry and livestock producers, examples of these substances include: zearalenone, H-2 and T-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol.

Zearalenone is one of the main toxins that is responsible for causing abortion, infertility and other bleeding problems. It has also been found to permeate through the human skin, however, there have been no significant hormonal effects which have been identified in normal residential and agricultural environments. When a large quantity of zearalenone contaminated feed is taken up by cows, this can be found to be detected in their milk, there is also suggestions that it can also be found in beer. It is regarded as the main contaminants of farm products, which can be subsequently consumed by humans or even other animals.

INTENDED USE

Zearalenone ELISA kit is designed for measuring rapid quantitative levels of zearalenone (ZEN) contaminations present in cereal and beer/gyle. This assay has a minimum analytical sensitivity limit of 5.0 ppb.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter plate: Coated with antibody binding protein.
Zearalenone Standards 1-6: Concentration 0, 10, 25, 75, 200, 500 ppb.
Conjugate (Zearalenone-Peroxidase).
Substrate Solution (TMB).
Stop Solution (1 N acidic solution).
Sample Diluent (PBS).
Washing Solution (PBS + Tween 20)(10x Concentrate).
Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of zearalenone contaminations using current zearalenone ELISA kit was 5.0 ppb. The standard range for this assay is 10.0 – 500.0 ppb.

ASSAY CHARACTERISTICS

– Sensitivity: Limit of detection, LOD (5ppb), Limit of quantification, LOQ (10ppb)
– Recovery: Oats flour (101%), Beer (100%), Rice flour, Corn flour (99%), Wheat flour (90%).
– Linearity: 82 – 102%
– Intra-Assay Precision: 4 – 7%
– Inter-Assay Precision: 5 – 13%
– Specificity (Cross Reactivity): Relative to Zearalenone (=100 %): α-Zearalenol (73%), α-Zearalanol (35%), β-Zearalenol (23%), β-Zearalanol (17%).

REFERENCES

Development of an immunochromatographic strip test for the rapid detection of zearalenone in corn. J Agric Food Chem. (2014) 62 (46): 11116-21. Sun Y., et al.
Zearalenone contamination in barley, corn, silage and wheat bran. Toxicol Ind Health. (2012) 28 (9): 779-82. Rashedi M., et al.
A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples. Toxins (Basel). (2015) 7 (10): 4216-31. Zhang X., et al.
A fluorescence polarization immunoassay for the detection of zearalenone in corn. Anal Chim Acta. (2009) 639 (1-2): 83-9. Chun H.S., et al.
Electrochemical microfluidic chips coupled to magnetic bead-based ELISAto control allowable levels of zearalenone in baby foods using simplified calibration. Analyst. (2009) 134 (12): 2405-11. Hervás M., et al.
Analysis of Fusarium toxins in maize and wheat using thin layer chromatography. Mycopathologia. (1998) 142 (2): 107-13. Schaafsma A.W., et al.

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