Aflatoxin B1 ELISA Kit

Full Name: Aflatoxin B1 ELISA Kit
Sample Type: Cereals, Beer/Gyle
Sensitivity: 0.5 ppb

BACKGROUND

Aflatoxin B1 is an incredibly potent carcinogen that is produced by Aspergillus flavus and A. parasiticus. However, this carcinogenic potency tends to vary across many different species for example rats displays a higher susceptibility when compared to monkeys. It can induce lung, colon and kidney carcinoma in dogs, rats, rodents and fish. The susceptibility is known to be age, sex and species dependent. Aflatoxin B1 induced mutations in the G-2448AGT polymorphism of the p53 gene, associated with increased risk of bladder cancer, are seen in human population populations exposed to high amounts through consumption of contaminated food stuffs such as maize, peanuts and rice. The primary route of exposure for humans is consumption of contaminated food stuffs such as maize, peanuts, rice, milk and eggs; while the route of exposure for birds is via feed contaminated with the fungal toxin.

Aflatoxin B1 is found in contaminated food such as; corn, cottonseed meal, peanuts and many other grains. Some of the common analytical procedure which have been used include HPLC, TLC, mass spectrometry and ELISA assays.

INTENDED USE

Aflatoxin B1 ELISA kit is a method for rapid quantitative measurement of aflatoxin B1 in beer/gyle and cereals. This assay has a minimum analytical sensitivity limit of 0.5 ppb.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Plate: Coated with antibody-binding protein.
Aflatoxin B1 Standards 1-6: Concentration 0, 1.5, 3, 6, 12, 24 ppb.
Anti-Aflatoxin Antibody (Rabbit).
Conjugate (Aflatoxin-Peroxidase).
Substrate Solution (TMB).
Stop Solution (0.5 M H2SO4).
Sample Diluent (PBS).
Washing Solution ( 10x Conc.).
Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of aflatoxin B1 using current aflatoxin B1 ELISA kit was 0.5 ppb. The standard range for this assay is 1.5 – 24.0 ppb.

ASSAY CHARACTERISTICS

– Sensitivity: Limit of detection (LOD) 0.5 ppb, limit of quantification (LOQ) 1.5 ppb.
– Recovery: Wheat flour (104%), Oats flour (86%), Rye flour (103%), Barley flour (98%), Rice flour (91%), Corn flour (94%), Beer (114%).
– Linearity: 82 – 115%
– Intra-Assay Precision: 3.0 – 6.0%
– Inter-Assay Precision: 5.0 – 11.0%
– Cross Reactivity: Relative to Aflatoxin B1 (=100%), Aflatoxin B2 (29%), Aflatoxin G1 (44%), Aflatoxin G2 (5%), Aflatoxin M1 (2%).

REFERENCES

Enzyme-linkedimmunosorbent (ELISA) determination of aflatoxin-B1 in peanut butter: collaborative trial. Food Addit Contam. (1988) 5 (4): 601-8. Mortimer D.N., et al.
Aflatoxins as a cause of hepatocellular carcinoma. J Gastrointestin Liver Dis. (2013) 22 (3): 305-10. Review. Kew M.C.
Effect of climate change on Aspergillus flavus and aflatoxin B1 production. Front Microbiol. (2014) 5: 348. Review. Medina A., et al.
Dietary aflatoxin exposure and chemoprevention of cancer: a clinical review. J Toxicol Clin Toxicol. (2003) 41 (2): 195-204. Review. Sudakin D.L.
A reviewof the dose-response induction of DNA adducts by aflatoxin_B1 and its implications to quantitative cancer-risk assessment. Mutat Res. (1993) 296 (3): 181-98. Review. Choy W.N.
Rapid immunoenzyme assayof aflatoxin B1 using magnetic nanoparticles. Sensors (Basel). (2014) 14 (11): 21843-57. Urusov A.E., et al.

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