- Created on the 15 February, 2017.
Beta-hCG ELISA kit is a reliable quantitative procedure for measuring beta human chorionic gonadotropin (beta hGC, beta-hCG) in serum samples. This assay has minimum sensitivity detection limit of 1.0 mIU/ml.
Beta human chorionic gonadotropin (beta-hCG) is essentially a pregnancy hormone that is produced by the cells of the implanting embryo. Beta hCG can be found to be detected in the maternal plasma or urine 8-9 following ovulation, then the levels are rapidly increased until it reaches a peak level by around 8-10 weeks, after this its levels start to decline gradually for the remainder of the pregnancy.
Human chorionic gonadotropin (hCG) has a molecular weight of 36,000 Da and is made up of 2 non-covalently bound subunits. The alpha subunit which approx. 10,205 Da molecular weight (“naked”) and is 92- amino acids in length, is identical in structure to thyroid-stimulating hormone (TSH), luteinizing hormone (LH) and follicle-stimulating hormone (FSH). The alpha subunit functions as the receptor transactivation portion. The different beta subunits are different for each of the above hormones, these display less homology and are responsible for conveying the receptor-specificity for each of the dimeric hormones. The differences in the beta subunit with each of the respective hormones is accountable for their biological specificity and immunochemical distinctiveness.
A large number of monoclonal antibodies which can recognise unique sites that are present on the beta chain of the ß-hCG/hCG molecule are essential used to differentiate between hCG and FSH, LH and TSH. There are many different assay’s that can be instrumental in the early detection of beta-hCG during pregnancy.
Reagents which are supplied with the beta-hCG ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: monoclonal anti-hCG antibody coated.
2. Standard (Standard 0-5). 0, 5; 25; 50; 100; 200 mIU/mL.
3. Sample Diluent.
4. Enzyme Conjugate.
5. TMB Substrate Solution.
6. Stop Solution.
The minimum sensitivity detection limit of beta human chorionic gonadotropin (anti-beta-hCG) using current beta-hCG ELISA kit was approximately 1.0 mIU/ml. The dynamic range for this assay is 5.0 – 200.0 mIU/ml.
- Immunoassay of human chorionic gonadotropin, its free subunits, and metabolites. Clin Chem. (1997) 43 (12): 2233-43. Review. Cole L.A.
- High level of beta-hCG simulating pregnancy in recurrent osteosarcoma: case report and review of literature. J Cancer Res Clin Oncol. (2004) 130 (6): 357-61. Review. Leidinger B., et al.
- Alpha-fetoprotein and beta-human chorionic gonadotropin: their clinical significance as tumour markers. Drugs. (1999) 57 (4): 463-7. Review. Gregory J.J. Jr and Finlay J.L.
- The hCG assay or pregnancy test. Clin Chem Lab Med. (2012) 50 (4): 617-30. Review. Cole L.A.
- Full Name: Beta Human Chorionic Gonadotropin (Beta-hCG) ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 1.0 mIU/ml