TSG101 ELISA Kit (Tumor Susceptibility Gene 101 Protein)

INTENDED USE

Human TSG101 ELISA kit can measure concentrations of tumor susceptibility gene 101 protein (TSG101, ESCORT-I complex) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

Tumor susceptibility gene 101 (TSG101) encodes a multifunctional protein best known for regulatory roles in cell growth, protein trafficking, and cancer progression when dysregulated. Identified originally as potential tumor suppressor in mammalian cells, subsequent insights revealed more complex functions dependent on cellular context. While frequently supporting cancer pathogenesis by enhancing proliferation and metastatic spread, TSG101 also serves vital roles in regulation of protein homeostasis through ubiquitin-mediated processing.

TSG101 participates in endosomal protein sorting as the central component of the ESCRT-I complex. By recognizing ubiquitinated membrane proteins marked for lysosomal degradation, ESCRT machinery clusters these cargo for involution into intraluminal vesicles budding into endosomes. TSG101 specifically binds ubiquitinated targets and recruits downstream ESCRTs enabling cargo capture and sorting into the MVB pathway terminating in lysosomal proteolysis. Thereby it critically maintains protein homeostasis by directing unwanted proteins towards degradation.

However in cancers, the same TSG101 activities enable growth-promoting effects and metastatic competence. By stimulating MVB formation, it enhances turnover of critical signaling proteins and receptors like epithelial growth factor receptor impacting downstream survival pathways. Furthermore, TSG101 overexpression in carcinomas promotes invasive behavior and intravasation. Hence, subversion of its protein trafficking functions in malignancy transforms this essential homeostatic regulator into a pathogenic enabler advancing disease severity.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-TSG101 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this TSG101 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TSG101, no cross reactivity or interference between TSG101 and analogues was detected.
– Recovery: Serum (93 – 100%), EDTA Plasma (85 – 105%), Heparin Plasma (87 – 98%).
– Linearity: Serum (87 – 95%), EDTA Plasma (82 – 100%), Heparin Plasma (85 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. TSG101 negatively regulates mitochondrial biogenesis in axons. Proc Natl Acad Sci U S A. (2021) 118 (20): e2018770118. Lin T.H., et al.
2. The Multifaceted Roles of the Tumor Susceptibility Gene 101 (TSG101) in Normal Development and Disease. Cancers (Basel). (2020) 12 (2): 450. Ferraiuolo R.M., et al.
3. Novel Tsg101 Binding Partners Regulate Viral L Domain Trafficking. Viruses. (2021) 13 (6): 1147. Strickland M., et al.
4. Tsg101/ESCRT-I recruitment regulated by the dual binding modes of K63-linked diubiquitin. Structure. (2022) 30 (2): 289-299.e6. Strickland M., et al.

ADDITIONAL INFORMATION

• Full Name: TSG101 ELISA Kit (Tumor Susceptibility Gene 101 Protein)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 18.75 pg/ml

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