Serotonin Research ELISA Kit (5-Hydroxytryptamine)

INTENDED USE

Serotonin research ELISA kit can be used for measuring in vitro quantitative levels of serotonin (5-hydroxytryptamine) human tissue homogenates, dialysates and many other biological sample types. This assay has a minimum detection limit of 0.005 ng/ml.

BACKGROUND

Serotonin (also called 5-hydroxytryptamine), is a biogenic amine that is formed for the metabolism of tryptophan. Around 80% that is present in humans is found in the gastrointestinal tract and this is located on enterochromaffin cells. The main function is to regulate the movement of the gut.

Serotonin is considered as a natural mood stabiliser and the chemical which is responsible for helping in the process of eating, digesting and sleeping. It is also found to help reduce depression, heal wounds, maintain bone health, stimulate nausea and regulate anxiety. Pharmacological antidepressants can be used in order to modulate serotonin that can be present at the synapses junction.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Standards (A-F): Concentrations 0, 0.015, 0.05, 0.15, 0.5, 2.5 ng/ml
• Control 1+ Control 2.
• Pre-Coated Serotonin Microtiter Strips.
• Enzyme Conjugate.
• Substrate (TMB).
• Serotonin Antiserum.
• Wash Buffer (50x Conc.).
• Stop Solution.
• Stabilizer: Stabilizing agent.
• Dilution Concentrate (20x).
• Acylation Plate.
• Acylation Buffer.
• Adhesive Foil.

SENSITIVITY

The minimum detection sensitivity level of serotonin (5-hydroxytryptamine) using current serotonin research ELISA kit was approximately 0.005 ng/ml. The dynamic range for this assay is 0.015 – 2.5 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity:  0.005 ng/ml x C* (C* = correction factor).
– Analytical Sensitivity (Cross Reactivity): Serotonin (100%), Tryptamine (0.19%), Melatonin (0.03%), Phenylalanine, 5-Hydroxytryptophane, Tyramine, 5-Hydroxyindole acetic acid, Histidine (< 0.002%).

REFERENCES

1. Serotonin-regulates performance nonmonotonically in a spatial working memory network. Cereb Cortex. (2014) 24 (9): 2449-63. Cano-Colino M., et al.
2. Oxidation of high doses of serotonin favors lipid accumulation in mouse and human fat cells. Mol Nutr Food Res. (2013) 57 (6): 1089-99. Grès S., et al.
3. The role of serotonin_in memory: interactions with neurotransmitters and downstream signaling. Exp Brain Res. (2014) 232 (3): 723-38. Review. Seyedabadi M., et al.
4. Changes in markers of brain-serotonin activity in response to chronic exercise in senior men. Appl Physiol Nutr Metab. (2014) 39 (11): 1250-6. Melancon M.O., et al.

ADDITIONAL INFORMATION

• Full Name: Serotonin Research ELISA Kit (5-Hydroxytryptamine)
• Reactivity: Human
• Sample Type: Tissue Homogenate, Dialysate, Other Biological Samples
• Sensitivity: 0.005 ng/ml

OTHER RELATED ELISA KITS

• Histamine Research ELISA Kit
• Tryptophan ELISA Kit
• Chromogranin A ELISA Kit