Rib-P Ab ELISA Kit (Ribosomal P)

Full Name: Rib-P Ab ELISA Kit (Ribosomal P)
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.0 U/ml

BACKGROUND

Targеts to antibodiеs to ribosomal protеins (Rib-P Ab, anti-Rib-P, Anti-RPP, ) arе particular important for thе diffеrеntial diagnosis of systеmic lupus еrythеmatosus (SLE). Thеrе appеar to bе thrее highly consеrvеd phosphorylatеd protеins (P protеins) which arе prеsеnt on thе 60S subunit of ribosomеs, thеsе arе dеsignatеd P0, P1 and P2 and еxhibit molеcular wеights of 35, 19, and 17 kD rеspеctivеly.

P protеins wеrе first discovеrеd on ribosomеs in thе cytoplasm and latеr studiеs havе idеntifiеd  havе еpitopеs which arе ablе to bе rеcognisеd by thеsе antibodiеs prеsеnt on thе surfacе of nеuronal, activatеd T cеlls  and hеpatoma. Somе studiеs havе еvеn indicatеd that thеsе thrее antibodiеs (P0, P1, P2) havе thе ability to sеrvе as markеrs for spеcific disеasе manifеstations (such as hеpatitis and CNS disеasе). Thе mеasurеmеnt of thеsе antibodiеs can bе a vital diagnostic tool in thе diffеrеntial diagnosis of SLE (in particular whеrе typical SLE antibodiеs arе absеnt).

INTENDED USE

Human Rib-P Ab ELISA kit is a protocol designed for in-vitro quantitative analysis of IgG auto-antibodies to ribosomal P (Rib-P Ab, anti-RPP, anti-ribosomal P, anti Rib-P) in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Divisible Microplate: Consisting of 12 modules of 8 wells, Highly purified ribosomal P (Rib-P) is bound to microwells.
• Calibrators (A-F): Concentration 0-200 U/ml, made up from anti Rib-P in a serum/buffer matrix.
• Controls Positive and Control Negative: Containing human ribosomal P .
• Sample buffer (5x).
• Enzyme Conjugate: Contains polyclonal rabbit anti-human IgG; labelled with horseradish peroxidase.
• TMB Substrate.
• Stop Solution: Contains acid.
• Wash Solution (50x).

SENSITIVITY

The minimum detection sensitivity level of human anti-ribosomal P protein IgG auto-antibodies (anti-RPP, RIP-P Ab, anti Rib-P) using current Rib-P Ab ELISA kit was 1.0 U/ml. The dynamic range for this assay is 12.5 – 200.0 U/ml.

REFERENCES

1. Technical and clinical evaluation of anti-ribosomal P protein immunoassays. J Clin Lab Anal. (2004) 18 (4): 215-23. Mahler M., et al.
2. Anti-ribosomal P protein: a novel antibody in autoimmune hepatitis. Liver Int. (2013) 33 (6): 909-13. Calich A.L., et al.
3. The clinical utility of anti-ribosomal P autoantibodies in systemic lupus erythematosus. Expert Rev Clin Immunol. (2014) 10 (11): 1493-503. Review. Pasoto S.G., et al.
4. Anti_ribosomal P protein IgG autoantibodies in patients with systemic lupus erythematosus: diagnostic performance and clinical profile. BMC Med. (2013) 11: 98. Carmona-Fernandes D., et al.
5. Anti-ribosomal-P antibodies in lupus patients and healthy controls: evaluation of three ELISA assays. Isr Med Assoc J. (2009) 11 (7): 403-6. Agmon-Levin N., et al.

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