RSV IgA ELISA Kit (Respiratory Syncytial Virus)

Full Name: RSV IgA ELISA Kit (Respiratory Syncytial Virus)
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.7 U/ml


Respiratory syncytial virus (RSV) is a syncytial virus which causes infections of the respiratory tract and lungs. It is a single stranded, negative sense RNA virus which belongs to the Pneumoviridae family. It is an approx. 120–200 nm enveloped virus which contains a linear negative-sense RNA genome and a lipoprotein coat. There are four viral genes which code for intracellular proteins that are involved in processes such as replication, transcription and particle budding. These are called matrix protein (M), “large” protein (L), Nucleoprotein (N) and phosphoprotein (P). The genome itself is about 15,000 nucleotides in length and is made up of single stranded RNA (displaying negative polarity) and it contains 10 genes in total which encode 11 proteins.

RSV is mainly transmitted through large droplets and also by secretions from contact with any infected people. The virus has an incubation period of 3-5 days before the symptoms begin to appear. ELISA assays play an important role for the serological diagnosis of RSV infections because of their sensitivities, specificities and the ability to allow differentiation of antigens into the various immunoglobulin classes.


Human RSV IgA ELISA kit is intended for determining in-vitro levels of human IgA antibodies against respiratory syncytial virus (RSV IgA) in serum or plasma. This assay has a minimum analytical sensitivity limit of 1.7 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • RSV antigen coated microtiter strips.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Sample Diluent.
  • Washing Buffer (10x).
  • Stop Solution.
  • Calibrators (A-D): Negative, Cut-Off, Weak Positive and Positive Controls.


The minimum detection sensitivity level of human IgA antibodies to respiratory syncytial virus (RSV-IgA) using current RSV IgA ELISA kit was 1.7 U/ml. The dynamic range for this assay is 1.0 – 100.0 U/ml.


– Intra-Assay-Precision: 10.1 %
– Inter-Assay-Precision: 6.8 %
– Inter-Lot-Precision: 1.0 – 8.7 %
– Analytical Sensitivity: 1.70 U/mL
– Recovery: 89 – 129 %
– Linearity: 87 – 126 %
– Cross-Reactivity: No cross-reactivity to Parainfluenza 1/2/3, Adenovirus and Influenza A.
– Interferences: No interferences to hemoglobin up to 8.0 mg/mL, triglycerides up to 5.0 mg/mL and bilirubin up to 0.3 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 86 %


  1. Immunoglobulin A antibody response to respiratory syncytial virus structural proteins in colostrum and milk. J Clin Microbiol. (1989) 27 (9): 1949-51. Tsutsumi H., et al.
  2. Association between pronounced IgA response in RSV bronchiolitis and development of allergic sensitization. Pediatr Allergy Immunol. (1997) 8 (1): 1-6. Strannegård O., et al.
  3. Vaccination with the RSV fusion protein formulated with a combination adjuvant induces long-lasting protective immunity. J Gen Virol. (2014) 95 (Pt 5): 1043-54. Garg R., et al.
  4. A single intranasal immunization with a subunit vaccine formulation induces higher mucosal IgA production than live respiratory syncytial virus. Virology. (2016) 499: 288-297. Garg R., et al.


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