MR-proANP ELISA Kit (Midregional pro-Atrial Natriuretic Peptide)
INTENDED USE
Human MR-proANP ELISA kit can measure concentrations of midregional pro-atrial natriuretic peptide (MR-proANP) present is plasma, serum, biological fluids or tissue homogenate samples.
INTRODUCTION
MR-proANP refers to mid-regional pro-atrial natriuretic peptide, a fragment of the prohormone peptide secreted alongside atrial natriuretic peptide (ANP) hormone from cardiac atria. As heart atrial stretch triggers secretion, it closely mirrors ANP release, serving as a biomarker gauging hemodynamic stress. Quantifying circulating MR-proANP levels assists clinical diagnosis, prognostication, and therapeutic monitoring across various acute and chronic cardiac pathologies ranging from congestive heart failure to coronary artery disease and infective endocarditis.
Within atrial cardiomyocytes, proANP hormone is stored as an inactive 126 amino acid propeptide that requires cleavage into active C-terminal ANP hormone plus residual N-terminal by-product MR-proANP upon secretion. This splitting is executed by the protease corin, which activates the natriuretic, diuretic and vasoactive ANP hormone in equimolar tandem production of MR-proANP. However, it exhibits substantially greater stability over ANP alone, with circulating concentrations integrating both biosynthesis and release rates by cardiomyocytes proportional to atrial stretch and pressure overload.
As such, immunoassays quantifying MR-proANP levels in plasma provide a robust biomarker closely estimating ANP alongside cardiac stress status. Elevated levels are associated with higher risks of mortality and rehospitalization in acutely decompensated and chronic heart failure patients. It also predicts adverse events in coronary artery disease while dropping appropriately following infarction, bypass grafting or alcohol ablation confirming therapy efficacy. Hence, MR-proANP measurements assist precision diagnosis and management across cardiac diseases tied to volume overload, inflammation, and tissue hypoxia reflecting atrial stress.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti- MR-proANP.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this MR-proANP ELISA kit, it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for MR-proANP, no cross reactivity or interference between MR-proANP and analogues was detected.
– Recovery: Serum (87 – 102%), EDTA Plasma (85 – 102%), Heparin Plasma (90 – 102%).
– Linearity: Serum (85 – 93%), EDTA Plasma (92 – 101%), Heparin Plasma (84 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%
REFERENCES
- MR-proANP measured at admission is associated with incident atrial fibrillation in STEMI patients. Heart Vessels. (2022) 37 (11): 1906-1913. Wegener A., et al.
- Biomarkers and diagnostics in heart failure. Biochim Biophys Acta. (2013) 1832 (12): 2442-50. Gaggin H.K.and Januzzi J.L.
- Elevated MR-proANP plasma concentrations are associated with sepsis and predict mortality in critically ill patients. J Transl Med. (2019) 17 (1): 415. Yagmur E., et al.
ADDITIONAL INFORMATION
- Full Name: MR-proANP ELISA Kit (Midregional pro-Atrial Natriuretic Peptide)
- Reactivity: Human
- Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
- Sensitivity: 0.094 ng/ml
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