Mollusc (tropomyosin) ELISA kit can be used for detecting quantitative levels of tropomyosin and mollusc in food (such as fish, meat, bakery products, soups and dressings). This assay has a minimum analytical sensitivity limit of 1.7 ppb.
Molluscs represent a large group of seafood’s which can include mussels, squid, snails, abalone, oysters, octopus, scallops, clams and squid. It is also a food allergen since a large number of adverse reactions have been reported following the ingestion of molluscs, the allergic reactions found are similar to those found with other foods. Tropomyosin is a vital protein which is common to all mollusc species, it represents a high amount of the total protein present for other cooked or uncooked mollusc extracts.
A very small quantity of allergen is enough cause allergic reactions and in severe cases can result in anaphylactic shock. There is also the possibility of cross-reactions between molluscs in particular within the same class (for example cephalopods, bivalves and gastropods). Allergies to molluscs tend to be diagnosed in a similar fashion to other food allergies.
MOLLUSC (TROPOMYOSIN) ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Coated with anti tropomyosin antibodies.
- Tropomyosin Standards 1-5: Concentration 0, 10, 40, 100, 400 ppb.
- Conjugate (Anti Tropomyosin Peroxidase).
- Substrate Solution (TMB).
- Stop Solution (0.5 M H2SO4).
- Extraction and sample dilution buffer (Tris)(10x).
- Washing Solution (PBS + Tween 20)(10x Concentrate).
- Instruction Manual.
The minimum detection sensitivity level of mollusc and tropomyosin using current mollusc/tropomyosin ELISA kit was 1.7 ppb. The standard range for this assay is 10.0 – 400.0 ppb.
– Mollusc (Tropomyosin): ELISA
– Sensitivity: Limit of detection, LOD (1.7ppb), Limit of quantification, LOQ (10.0ppb)
– Intra-Assay Precision: 5 – 6%
– Inter-Assay Precision: 3%
– Recovery: Meat (110%), Fish (103%), Bakery products (100%), Vegetable soup (88%), Soy sauce (74%).
– Linearity: 89 – 107%
– Specificity (Cross Reactivity): No cross reaction was detected for the following: Apricot, Potato, Plum, Bean (white), Chervil, Pine seed, Buckwheat, Chickpea, Pistachio, Cashew, Cherry, Rice, Cayenne, Kiwi, Beef, Chili, Coconut, Bovine gelatine, Cumin, Cress, Rye, Cod, Pumpkin seed, Saccharose, Egg, Caraway, Ewe’s milk, Pea, Lamb, Pork, Egg white powder, Lentil, Celery, Peanut, Lupin, Mustard, Fish gelatine, Corn, Sesame, Barley, Macadamia, Soy, Gliadin, Almond, Soy lecithin, Guar gum, Chestnut, Sunflower seed, Oats, Poppy seed, Tofu, Hazelnut, Nutmeg, Tomato, Isinglass, Bell pepper, Cow’s milk, Chicken, Brazil nut, Walnut, Carob gum, Pecan nut, Wheat, Cocoa, Pepper, Goat’s milk, Carrot, Peach, Onion.
- Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure. Food Chem Toxicol. (2015) 76: 86-93. Jin Y., et al.
- Effect of pH shifts on IgE-binding capacity and conformational structure of tropomyosin from short-neck clam (Ruditapes philippinarum). Food Chem. (2015) 188: 248-55. Lin H., et al.
- Application of rapid test kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain. Toxicon. (2016) 111: 121-9. Johnson S., et al.
- Cross-reactivity between storage and dust mites and between mites and shrimp. Exp Appl Acarol. (2009) 47 (2): 159-72. Arlian L.G., et al.
- A novel monoclonal antibody-based immunoenzymatic assay for epidemiological surveillance of the vector snails of Fasciola hepatica (Trematoda: Digenea). Int J Parasitol. (2015) 45 (2-3): 113-9. Alba A., et al.
- Full Name: Mollusc (Tropomyosin) ELISA Kit
- Sample Type: Food (Soups, Fish, Bakery, Dressings, Meat)
- Sensitivity: 1.7 ppb