Mollusc ELISA Kit (Tropomyosin)

Full Name: Mollusc ELISA Kit (Tropomyosin)
Sample Type: Food (Soups, Fish, Bakery, Dressings, Meat)
Sensitivity: 1.7 ppb

BACKGROUND

Molluscs represent a large group of seafood’s which can also include; mussels, squid, snails, abalone, oysters, octopus, scallops, clams and squid. They are a food allergen because molluscs consumption has resulted in a large number of adverse reactions. The allergic reactions found are similar to those found with other foods and with the other groups of seafood. Shellfish such as; mussels, oysters, clams, scallops and squid are filter feeders that remove food particles while they swim about in the water, are responsible for over 90% of seafood allergic reactions. The remining10% of reactions which do not involve shellfish are caused by fish and crustaceans such as shrimp and crab.

Tropomyosin is a vital protein which is common to all mollusc species, it represents a high amount of the total protein present for other cooked or uncooked mollusc extracts. A very small quantity is enough cause allergic reactions and in severe cases can result in anaphylactic shock. Mollusc allergies are typically diagnosed in the same way as other food allergies.

INTENDED USE

Mollusc ELISA kit can be used for detecting quantitative levels of tropomyosin and mollusc in food (such as fish, meat, bakery products, soups and dressings). This assay has a minimum analytical sensitivity limit of 1.7 ppb.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Plate: Coated with anti-tropomyosin antibodies.
Tropomyosin Standards 1-5: Concentration 0, 10, 40, 100, 400 ppb.
Conjugate (Anti-Tropomyosin-Peroxidase).
Substrate Solution (TMB).
Stop Solution (0.5 M H2SO4).
Extraction and sample dilution buffer (Tris)(10x).
Washing Solution (PBS + Tween 20)(10x Concentrate).
Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of mollusc and tropomyosin using current mollusc ELISA kit was 1.7 ppb. The standard range for this assay is 10.0 – 400.0 ppb.

ASSAY CHARACTERISTICS

– Sensitivity: Limit of detection, LOD (1.7ppb), Limit of quantification, LOQ (10.0ppb)
– Intra-Assay Precision: 5 – 6%
– Inter-Assay Precision: 3%
– Recovery: Meat (110%), Fish (103%), Bakery products (100%), Vegetable soup (88%), Soy sauce (74%).
– Linearity: 89 – 107%
– Specificity (Cross Reactivity): No cross reaction was detected for the following: Apricot, Potato, Plum, Bean (white), Chervil, Pine seed, Buckwheat, Chickpea, Pistachio, Cashew, Cherry, Rice, Cayenne, Kiwi, Beef, Chili, Coconut, Bovine gelatine, Cumin, Cress, Rye, Cod, Pumpkin seed, Saccharose, Egg, Caraway, Ewe’s milk, Pea, Lamb, Pork, Egg white powder, Lentil, Celery, Peanut, Lupin, Mustard, Fish gelatine, Corn, Sesame, Barley, Macadamia, Soy, Gliadin, Almond, Soy lecithin, Guar gum, Chestnut, Sunflower seed, Oats, Poppy seed, Tofu, Hazelnut, Nutmeg, Tomato, Isinglass, Bell pepper, Cow’s milk, Chicken, Brazil nut, Walnut, Carob gum, Pecan nut, Wheat, Cocoa, Pepper, Goat’s milk, Carrot, Peach, Onion.

REFERENCES

Allergenic response to squid (Todarodes pacificus) tropomyosin Tod p1 structure modifications induced by high hydrostatic pressure. Food Chem Toxicol. (2015) 76: 86-93. Jin Y., et al.
Effect of pH shifts on IgE-binding capacity and conformational structure of tropomyosin from short-neck clam (Ruditapes philippinarum). Food Chem. (2015) 188: 248-55. Lin H., et al.
Application of rapid test kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain. Toxicon. (2016) 111: 121-9. Johnson S., et al.
Cross-reactivity between storage and dust mites and between mites and shrimp. Exp Appl Acarol. (2009) 47 (2): 159-72. Arlian L.G., et al.
A novel monoclonal antibody-based immunoenzymatic assay for epidemiological surveillance of the vector snails of Fasciola hepatica (Trematoda: Digenea). Int J Parasitol. (2015) 45 (2-3): 113-9. Alba A., et al.

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