Indoleamine 2,3-Dioxygenase 1 ELISA Kit (IDO1)

INTENDED USE

Human IDO1 ELISA kit can measure concentrations of IDO1 (Indoleamine 2,3-Dioxygenase 1, IDO-1, 3-dioxygenase) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 0.094 ng/ml.

INTRODUCTION

Indoleamine 2,3 dioxygenase 1 (IDO1) is an enzyme, with effects. It plays a role in the step of tryptophan degradation along the kynurenine pathway. By reducing levels of tryptophan IDO1 acts as an immunosuppressant on T cells. Supports immune tolerance.

IDO1 can be found in tissues and white blood cells. It can be induced by signals related to inflammation such as interferons type I and II. During infection or inflammation IDO1 activity in antigen presenting cells like cells helps restrain T cell proliferation and activation in tissues. This effect occurs through the breakdown of tryptophan which’s an amino acid needed for T cell differentiation and expansion. The combination of tryptophan depletion and the production of apoptotic byproducts leads to a tolerant microenvironment that hampers T cell responses. Moreover high expression of IDO1 in antigen presenting cells is linked to increased populations of T cells that suppress inflammation.

In tumors IDO1 activity plays a role by promoting evasion and tolerance against anti tumor T cell responses. Many human cancers exhibit heightened levels of IDO1 expression, which is associated with infiltrating T cells and poorer prognosis. The catabolism of tryptophan mediated by IDO1 within tumor cells and tumor associated myeloid cells is crucial, for creating a tumor microenvironment that favors cancer progression. Inhibiting IDO1 could potentially reactivate tumor immunity.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- IDO1.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this IDO1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IDO1, no cross reactivity or interference between IDO1 and analogues was detected.
– Recovery: Serum (90 – 104%), EDTA Plasma (95 – 103%), Heparin Plasma (86 – 104%).
– Linearity: Serum (85 – 100%), EDTA Plasma (83 – 95%), Heparin Plasma (80 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. The role of indoleamine 2,3-dioxygenase in allergic disorders. Mol Biol Rep. (2022) 49 (4): 3297-3306. Esmaeili S.A. and Hajavi J.
2. IDO1 and inflammatory neovascularization: bringing new blood to tumor-promoting inflammation. Front Oncol. (2023) 13: 1165298. Muller A.J., et al.
3. Indoleamine 2,3-dioxygenase-2; a new enzyme in the kynurenine pathway. Int J Biochem Cell Biol. (2009) 41 (3): 467-71. Ball H.J., et al.
4. Impact of IDO1 and IDO2 on the B Cell Immune Response. Front Immunol. (2022) 13: 886225. Merlo L.M.F., et al.

ADDITIONAL INFORMATION

• Full Name: Indoleamine 2,3-Dioxygenase 1 ELISA Kit (IDO1)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.094 ng/ml

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