IL-17 A/F ELISA Kit (Interleukin 17A/F)

INTENDED USE

Human IL-17 A/F ELISA kit can measure concentrations of interleukin 17A/F (IL-17 A/F) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

Interleukin 17 (IL-17) refers to a family of inflammatory cytokines critical for antimicrobial immunity but also implicated in autoimmune pathology when dysregulated. The family contains six members (IL-17A through IL-17F), with IL-17A and IL-17F sharing the highest sequence homology. Initial discovery of this unique CD4+ T cell subset arose in the 1990s, when IL-17 induced cytokine was identified and named CTLA8. A few years later the source was confirmed as the novel T helper 17 (TH17) lineage, specialized in producing IL-17A and IL-17F upon activation by specific cytokines.

Specifically, IL-17 family cytokines trigger production of granulopoietic factors and neutrophil chemoattractants by tissue cells at barrier sites. This stimulates bone marrow release and recruitment of neutrophils while prolonging their lifespan – mobilizing rapid, sustained anti-microbial activity against extracellular pathogens during infection. Accordingly, deficiencies in TH17 development or IL-17 signaling significantly impair immunity to certain bacterial and fungal infections at mucosal surfaces. However, excessive or chronic IL-17 production can also precipitate inflammatory disease by driving tissue damage.

Dysregulated TH17 cell activity and heightened IL-17 signaling associate with several autoimmune conditions including multiple sclerosis, rheumatoid arthritis, psoriasis, and inflammatory bowel disease. Antibodies neutralizing IL-17A or its receptor show efficacy in treating chronic plaque psoriasis and ankylosing spondylitis in clinical trials, significantly limiting disease progression.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- IL-17 A/F.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this IL-17 A/F ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-17 A/F, no cross reactivity or interference between IL-17 A/F and analogues was detected.
– Recovery: Serum (90 – 104%), EDTA Plasma (93 – 103%), Heparin Plasma (85 – 105%).
– Linearity: Serum (88 – 105%), EDTA Plasma (83 – 96%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. The role of heterodimer IL-17-A/F in atopic dermatitis. Postepy Dermatol Alergol. (2022) 39 (6): 1093-1100. Krzysiek J., et al.
2. Biology of Interleukin-17 and Its Pathophysiological Significance in Sepsis. Front Immunol. (2020) 11: 1558. Ge Y., et al.
3. Critical role for IL-17A/F in the immunopathogenesis of obliterative airway disease induced by Anti-MHC I antibodies. Transplantation. (2013) 95 (2): 293-300. Basha H.I., et al.
4. Differential effects of interleukin-17A and 17F on cell interactions between immune cells and stromal cells from synovium or skin. Sci Rep. (2023) 13 (1): 19223. Tout I., et al.

ADDITIONAL INFORMATION

• Full Name: IL-17 A/F ELISA Kit (Interleukin 17A/F)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 18.75 pg/ml

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