IGSF11 ELISA Kit (Immunoglobulin Superfamily Member 11)
INTENDED USE
Human IGSF11 ELISA kit can measure concentrations of immunoglobulin superfamily member 11 (IGSF11, Bt-IGSF, CXADRL1, VSIG3) present is plasma, serum, biological fluids or tissue homogenate samples.
INTRODUCTION
IGSF11 is an understudied transmembrane protein predominantly expressed in the central nervous system. Initially discovered in 2006, it stands out for its atypical immunoglobulin-like domain and selective expression pattern, suggesting specialized functions within the brain. Despite over 15 years since its identification, remarkably little is definitively known about IGSF11’s physiological importance.
IGSF11 transcripts show enrichment in the developing mouse brain, especially the cortex, hippocampus, and cerebellum. Adult expression becomes more restricted to subsets of neurons in cortical layers 2 and 5, Purkinje cells, and cerebellar granule cells. At the subcellular level, it localizes to the somatodendritic plasma membrane compartment, positioning it for a potential role in cell-cell recognition or adhesion. Intriguingly, IGSF11 demonstrates differential splicing – generating distinct isoforms containing either 3 or 4 extracellular immunoglobulin domains.
More recent work begins illuminating IGSF11’s function by analyzing mouse knock-out models. Deletion of it causes no gross abnormalities or neuron loss, consistent with compensation by other proteins during development. However subtle synaptic defects emerge in cortical and hippocampal neurons including alterations in dendrite spine stability and morphology. Parallel behavioral evaluations reveal impaired motor learning and memory consolidation. Taken together, results implicate postsynaptic IGSF11 in mediating structural and functional plasticity.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti- IGSF11.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this IGSF11 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 78.125, 156.25, 312.5, 625, 1250, 2500, 5000 pg/ml
Reactivity: Human
Sensitivity: 46.875 pg/ml
Range: 78.125 – 5000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for IGSF11, no cross reactivity or interference between IGSF11 and analogues was detected.
– Recovery: Serum (85 – 96%), EDTA Plasma (89 – 102%), Heparin Plasma (89 – 102%).
– Linearity: Serum (88 – 98%), EDTA Plasma (87 – 101%), Heparin Plasma (81 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%
REFERENCES
- IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene. PLoS Genet. (2021) 17 (9): e1009778. Chen B., et al.
- IgSF11-mediated phosphorylation of pyruvate kinase M2 regulates osteoclast differentiation and prevents pathological bone loss. Bone Res. (2023) 11 (1): 17. Kim H., et al.
- Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity. Nat Neurosci. (2016) 19 (1): 84-93. Jang S., et al.
ADDITIONAL INFORMATION
- Full Name: IGSF11 ELISA Kit (Immunoglobulin Superfamily Member 11)
- Reactivity: Human
- Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
- Sensitivity: 46.875 pg/ml
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