Flotillin-1 ELISA Kit (FLOT1)

INTENDED USE

Human flotillin-1 ELISA kit can measure concentrations of signal transducer and activator of FLOT1 (flotillin-1) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

The FLOT1 gene encodes the 427 amino acid flotillin-1 protein, which is broadly expressed across human tissues but found at especially high levels in brain, heart, and hematopoietic cells. It contains a flotillin domain through which it can form oligomeric complexes with the related protein flotillin-2 within lipid rafts. Flotillin oligomerization helps stabilize lipid rafts and maintain their structural integrity. Flotillins also directly bind cholesterol and are implicated in cholesterol uptake and trafficking processes. Through these mechanisms, flotillins create specialized platforms that facilitate receptor recruitment into rafts and assembly of signaling molecules following receptor activation.

It is one of the major proteins found in lipid rafts – specialized areas within the cell membrane that contain high concentrations of cholesterol and glycolipids. flotillin-1 directly interacts with a variety of cell surface receptors and helps scaffold downstream signaling molecules.

Because flotillin is a key scaffolding protein within lipid rafts, it likely exerts functional effects on diverse cellular processes including cytoskeletal dynamics, membrane curvature and trafficking events, cell adhesion, and activation of proliferation pathways like MAPK signaling. While the precise regulatory roles of flotillin-1 remain to be fully characterized for many pathways, altered FLOT-1 expression has been reported in several cancers, and the protein may prove to have broad utility as a biomarker or therapeutic target.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-FLOT1 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this flotillin-1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for FLOT1, no cross reactivity or interference between FLOT1 and analogues was detected.
– Recovery: Serum (89 – 103%), EDTA Plasma (86 – 103%), Heparin Plasma (85 – 105%).
– Linearity: Serum (88 – 103%), EDTA Plasma (85 – 96%), Heparin Plasma (80 – 94%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Flotillin-1 enhances radioresistance through reducing radiation-induced DNA damage and promoting immune escape via STING signaling pathway in non-small cell lung cancer. Cancer Biol Ther. (2023) 24 (1): 2203332. Wang Y., et al.
2. EIF4A3 Acts on the PI3K-AKT-ERK1/2-P70S6K Pathway through FLOT1 to Influence the Development of Lung Adenocarcinoma. Mol Cancer Res. (2023) ;21 (7): 713-725. Yu W., et al.
3. Clinical significance and comparison of flotillin 1 expression in left and right colon cancer. Oncol Lett. (2019) 18 (2): 997-1004. Baig N., et al.
4. Dynamics and Endocytosis of Flot1 in Arabidopsis Require CPI1 Function. Int J Mol Sci. (2020) 21 (5): 1552. Cao Y., et al.

ADDITIONAL INFORMATION

• Full Name: Flotillin-1 ELISA Kit (FLOT1)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.094 ng/ml

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