Fibrinogen ELISA Kit (FG)
INTENDED USE
Human fibrinogen ELISA kit can measure concentrations of FG (fibrinogen) present is plasma, serum, biological fluids or tissue homogenate samples.
INTRODUCTION
Fibrinogen comprises a family of large plasma glycoproteins synthesized in the liver which play a central role in blood clotting, fibrinolysis, cellular and matrix interactions, inflammation, wound healing, and neoplasia. Encoded by three genes labeled FGA, FGB, and FGC, fibrinogen is secreted as a soluble 340 kDa heteromultimer containing two sets of three distinct polypeptide chains (α, β, and γ).
Following vascular injury, thrombin-mediated cleavage of fibrinopeptide fragments from fibrinogen leads to spontaneous polymerization into fibrin fibrils, forming an insoluble gel that stabilizes the primary hemostatic platelet plug. Crosslinking by factor XIII further reinforces clot mechanical stability. Meanwhile, binding of fibrin to integrins, growth factors, and extracellular matrix proteins triggers downstream signaling involved in inflammation and epithelialization phases of wound repair. Elevated plasma fibrinogen predisposes towards thrombosis and associates with cardioembolic risk, while hypofibrinogenemia manifests in bleeding disorders.
In addition to coagulation, fibrin(ogen) molecules play important scaffolding roles within tumors, supplying a temporary matrix promoting growth, adhesion, and migration. Fibrinogen uptake and peri-tumoral deposition further stimulates angiogenesis. Normalized after surgical resection, high pre-operative fibrinogen predicts metastases and poorer prognosis across malignancies—including lung, prostate, cervical, and renal cancers. Evaluating fibrinogen/fibrin pathways as modifiers of tumor progression and markers of occult metastases may enhance therapeutic planning and patient outcomes. New anticoagulants targeting upstream thrombin activity rather than fibrinogen itself, may offer clinical benefit by minimizing coagulation-independent tumor promotion.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-FG antibody.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this fibrinogen ELISA kit, it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 1.562, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml.
Reactivity: Human
Sensitivity: 0.938 ng/ml
Range: 1.563 – 100 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for FG, no cross reactivity or interference between FG and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (90 – 104%), Heparin Plasma (89 – 103%).
– Linearity: Serum (86 – 100%), EDTA Plasma (86 – 96%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%
REFERENCES
- Fibrinogen and factor XIII at the intersection of coagulation, fibrinolysis and inflammation. Thromb Haemost. (2014) 112 (4): 649-58. Hoppe B.
- Congenital (hypo-)dysfibrinogenemia and bleeding: A systematic literature review. Thromb Res. (2022) 217: 36-47. Li Y., et al.
- Structure and function of fibrinogen inferred from hereditary dysfibrinogens. Int J Hematol. (2000) 72 (4): 436-47. Matsuda M.
- The normal and morbid biology of fibrinogen. Am J Med. (1989) 87 (5): 567-76. Dang C.V., et al.
ADDITIONAL INFORMATION
- Full Name: Fibrinogen ELISA Kit (FG)
- Reactivity: Human
- Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
- Sensitivity: 0.938 ng/ml
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