Anti-Centromere B ELISA Kit
INTENDED USE
Human anti-centromere B ELISA kit is designed for determining in vitro quantitative concentrations of anti IgG autoantibodies against anti centromere B antibody in human serum and plasma. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.
BACKGROUND
Anti-centromere antibodies (which can be also called ACAs or anti-centromere) are essential autoantibodies which are highly specific to kinetochore and centromere functions. They are predominately identified in systemic scleroderma (also more commonly referred to as CREST syndrome) and in some cases present in scleroderma (diffused form). The symptoms displayed by CREST patients are: Calcinosis (deposits of calcium under the skin), Raynaud (reduced flow rate of blood to toes and fingers, leading to them turning blue and white), Esophageal dysfunction (acid reflux, heartburn and swallowing difficulty), Sclerodactyly (shiny, thick and tight skin on the fingers and hands), Telangiectasia (presence of red spots on the skin because of swollen capillaries).
The detection of autoantibodies which are directed against centromere protein B has become very important for the diagnostic and prognostic of progressive systemic scleroderma (PSS). Centromere protein B is a DNA-associated protein and together with other proteins, is vital in the formation of kinetochores. It is an essential target antigen for over 95% of patients that have Raynaud’s phenomenon and scleroderma. Since majority of autoantibodies against centromere proteins are present many year before the disease appears, this makes the detection of anti-centromere B a significant indicator for the onset of scleroderma.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Divisible Microplate: Consisting of 12 modules of 8 wells each, recombinant anti centromere B is bound to microwells.
- Calibrator A-E: Concentration 0, 10, 30, 100, 300 U/ml.
- Control Positive And Control Negative.
- Sample Buffer (5x).
- Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
- Substrate: Tetramethylbenzidine (TMB).
- Stop Solution: Contains acid.
- Wash Buffer (50x).
- Instruction for Use.
- Certificate of Analysis.
SENSITIVITY
The minimum detection sensitivity level of human centromere B antibody (anti centromere B) using current anti-centromere B ELISA kit was 1.0 U/ml. The dynamic range for this ELISA assay is 10.0 – 300.0 U/ml.
ASSAY CHARACTERISTICS
– Sensitivity: 1.0 U/ml.
– Intra Assay: 4.4 – 5.4%
– Inter Assay: 4.2 – 5.4%
REFERENCES
- Sensing centromere tension: Aurora B and the regulation of kinetochore function. Trends Cell Biol. (2011) 21 (3): 133-40. Review. Lampson M.A. and Cheeseman I.M.
- DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function. Dev Cell. (2015) 33 (3): 314-27. Fachinetti D., et al.
- Discovering centromere proteins: from cold white hands to the A, B, C of CENPs. Nat Rev Mol Cell Biol. (2015) 16 (7): 443-9. Review. Earnshaw W.C.
- The role of CENP-B and alpha-satellite DNA: de novo assembly and epigenetic maintenance of human centromeres. Chromosome Res. (2004) 12 (6): 543-56. Review. Masumoto H., et al.
- Building the centromere: from foundation proteins to 3D organization. Trends Cell Biol. (2004) 14 (7): 359-68. Review. Amor D.J., et al.
ADDITIONAL INFORMATION
- Full Name: Anti-Centromere ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 1.0 U/ml
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