ALDOA ELISA Kit (Aldolase A, Fructose Bisphosphate)

INTENDED USE

Human ALDOA ELISA kit can measure concentrations of aldolase A, fructose Bisphosphate (ALDOA) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

As a tetrameric glycolytic enzyme catalyzing fructose-1,6-bisphosphate cleavage into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, fructose-bisphosphate aldolase A (ALDOA) holds a critical role regulating glucose metabolism and ATP generation through both catabolic and anabolic pathways. Mammalian aldolase exists as three isozyme subtypes with 65%-68% amino acid identity that differ in kinetic behavior, immunoreactivity, and predominant tissue expression patterns: ALDOA in muscle, ALDOB in liver, and ALDOC in brain.

While metabolically fundamental across cell types, ALDOA demonstrates the highest levels in adult striated muscle, consistent with the elevated glycolytic poise supporting contractile activity in these tissues. Beyond the cytosol, it also occupies non-canonical nuclear and sarcomeric locales. Through transcriptional cofactor functions or direct modulation of filament assembly dynamics, extracellular ALDOA further coordinates adaptive responses to hypoxic, oxidative, or mechanical stress. Dysregulation of ALDOA links with hereditary myopathies and glycogen storage disorders stemming from impaired muscle energy homeostasis.

Cancer cells share the upregulated glycolytic phenotype of contracting muscle, often with disproportionate elevation of ALDOA reflecting glycolytic dependence even under normoxic conditions. Thus, inhibiting elicits cytotoxic actions across numerous solid and hematological malignancies by triggering ATP crisis, oxidative damage, and apoptosis—particularly efficacious against metastasis. Impacts on cytoskeletal architecture may further mediate ALDOA blockade. Advancing knowledge of molecular switches dictating tissue-specific functionality should facilitate optimization of therapeutic targeting strategies.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-ALDOA antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this ALDOA ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml.
Reactivity: Human
Sensitivity: 37.5 pg/ml
Range: 62.5 – 4000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for ALDOA, no cross reactivity or interference between ALDOA and analogues was detected.
– Recovery: Serum (89 – 105%), EDTA Plasma (88 – 97%), Heparin Plasma (93 – 104%).
– Linearity: Serum (86 – 104%), EDTA Plasma (88 – 101%), Heparin Plasma (89 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. ALDOA maintains NLRP3 inflammasome activation by controlling AMPK activation. Autophagy. 2022 Jul;18(7): 1673-1693. Bai D., et al.
2. The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity. Oncogene. (2022) 41 (7): 1024-1039. Lin J., et al.
3. Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome. Nat Methods. (2022) 19 (7): 854-864. Wan N., et al.
4. A novel lncRNA ARST represses glioma progression by inhibiting ALDOA-mediated actin cytoskeleton integrity. J Exp Clin Cancer Res. (2021) 40 (1): 187. Sun J., et al.

ADDITIONAL INFORMATION

• Full Name: ALDOA ELISA Kit (Aldolase A, Fructose Bisphosphate)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 37.5 pg/ml

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