Substance P ELISA Kit (SP)

INTENDED USE

Human substance P ELISA kit can measure concentrations of SP (substance P) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

Substance P is an undecapeptide that functions as a neurotransmitter and neuromodulator involved in transmitting signals for pain, stress, anxiety, nausea and inflammation. It belongs to the tachykinin family of peptides sharing a common C-terminal sequence. In the central and peripheral nervous system, substance P is produced from the preprotachykinin-A (PPT-A) gene and released from neuron endings to bind receptors including the neurokinin-1 receptor (NK1R).

As a key transmitter of nociception, substance P regulates pain signaling. Noxious stimuli trigger its release from primary sensory afferents, communicating with dorsal horn neurons of the spinal cord that modulate central pain pathways. This sensitizes second-order neurons, inducing hyperexcitability that amplifies pain. Its production and release further escalate in inflamed tissues. Consequently, substance P elicits neurogenic inflammation, directly stimulating immune cells and blood vessels to exacerbate swelling, vasodilation, and pain hypersensitivity. It likewise stimulates release of pro-inflammatory cytokines including histamine, serotonin, and prostaglandins.

Given its dominant roles in pain and neuroinflammation, substance P and its preferred NK1 receptor have emerged as promising therapeutic targets. NK1R antagonists help treat nausea, emesis, depression, and anxiety.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-Substance P antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this substance P ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml.
Reactivity: Human
Sensitivity: 4.688 pg/ml
Range: 7.813 – 500 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for substance P, no cross reactivity or interference between substance P and analogues was detected.
– Recovery: Serum (88 – 105%), EDTA Plasma (86 – 105%), Heparin Plasma (85 – 99%).
– Linearity: Serum (85 – 105%), EDTA Plasma (89 – 105%), Heparin Plasma (87 – 105%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Substance P in cardiovascular diseases – A bioanalytical review. Clin Chim Acta. (2019) 495: 501-506. Feickert M. and Burckhardt B.B.
2. Substance P–a putative mediator of antidromic vasodilation. Gen Pharmacol. (1983) 14 (1): 13-6. Pernow B.
3. Substance P: its pharmacology and physiological roles. Aust J Exp Biol Med Sci. (1977) 55 (6): 671-735. Bury R.W. and Mashford M.L.
4. Intraneuronal substance P contributes to the severity of experimental arthritis. Science. (1984) 226 (4674): 547-9. Levine J.D., et al.

ADDITIONAL INFORMATION

• Full Name: Substance P ELISA Kit (SP)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 4.688 pg/ml

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