ELISA kit method is a widely used and well proven solid-phase immunoassay technique which can detect or quantify one or several different analytes in an aqueous sample. It is useful in helping provide accurate, sensitive and consistent results, where each target protein is researched and each kit is calibrated to provide physiologically relevant sensitivity.
FEATURES AND BENEFITS OF OUR ELISA KIT
- Broad range of targets available over 2,000 at the moment and we are constantly adding more.
- Optimised for accurate, sensitive and consistent performance. They are put through rigorous quality control specifications and are manufactured in ISO accredited facilities in order to help ensure excellent quality and reproducibility.
- Validated for sample type and for species specificity, thereby providing a physiologically relevant sensitivity.
- Contains all the reagents and solutions required to perform the ELISA experiment including the protein standards and controls.
- It is flexible and can be adapted to be used on an automated equipment in order for higher throughput systems.
- A detailed protocol insert with an easy step by step instructions guide is provided.
TYPICAL CONTENTS OF AN ELISA KIT
- Antibody-coated 96-well plate or 96-well pre-coated strip plate (12×8). Usually a polystyrene solid support which can be stored for 6-10 months at room temperature (2-8°C).
- Standards and controls. Provided either in a pre-diluted format or may require some dilution. These need to be included with each plate so the results are valid and meaningful.
- Primary detection antibody (typically biotinylated) and a secondary detection reagent (usually HRP-streptavidin). Key component of the assay, a highly purified IgG detection antibody is recommended and the preferred enzyme reagent used is either horseradish peroxidase (HRP) or alkaline phosphatase (AP).
- Sample diluent buffers and wash buffer concentrate (20x). Need to bring both these reagents to room temperature before using them.
- Substrate and stop solution. Substrate solution needs to be kept in the dark until required for use, since any expose to light could affect its chemical activity. The stop solution needs to be bought to room temperature before use, it also needs to be clear without any crystals present.
- Plate cover.
- Instruction manual.
AN OVERVIEW OF THE ELISA KIT METHOD
- A typical sandwich ELISA method employ specific capture antibodies that have been pre-coated on a 96-well plate.
- Standards and samples are then pipetted into each of the specified wells, this will result in the target protein in the standards and samples binding to the immobilized antibody.
- The wells are then thoroughly washed (usually using PBS) in order to remove any non-specific binding, followed by the addition of the biotin-labelled detection antibody.
- After a second washing step to remove away any unbound biotinylated antibodies, AP or HRP-conjugated streptavidin is pipetted into each well, followed by a colorimetric substrate solution. Finally a stop solution is added to each well in order to end the reaction.
- A standard curve is prepared using the data obtained from the standards and this is used to interpolate the concentration of the samples.
- The intensity of colour that develops in each of the wells is proportional to the amount of target protein bound.
ELISA KITS PRODUCT CATALOG
- Autoimmunity ELISA Kits
Some of the most common diagnostic markers used to detect autoimmune diseases include: anti-CCP, ANA and ENA for rheumatic diseases, cardiolipin and ß2-GP1 for vasculitis diseases, anti-DGP IgG or anti-DGP IgA and tTG for celiac diseases, TPO and anti-TRAb for thyroid disesaes.
- Biogenic Amines ELISA Kits
Five of the most common biogenic amines include: epinephrine (adrenaline), norepinephrine (noradrenaline), serotonin, histamine and dopamine. Other useful biogenic amines are GABA, glutamate, nephrine, metanephrine, metanephrine, normetanephrine, tryphtophan and melatonin.
- Cardiac Markers ELISA Kits
Most frequently used cardiac markers to diagnose heart attach include: cardiac troponin (cTn-1 and TnT), creatinine kinase-MB (CK-MB) and myoglobin. Other useful biomarker include: c-reactive protein (CRP), ferritin, b-type natriuretic peptide (BNP), haptoglobin, homocysteine, cystatin and fibrinogen.
- Endocrinology Diagnostics ELISA Kits
Common topics under this branch of medicine include:
[A]. Thyroid Function ELISA: T3, T4, FT3, FT4, TG, hTG, TSH, TPO and TRAB.
[B]. Bone and Mineral Metabolism ELISA: Calcitonin, osteopontin, osteocalcin, PTH and 25-OH Vitamin D.
[C]. Nephrology ELISA: ACTH, aldosterone, angiotensin, b2MG, ET-1, Big ET-1, ET-2, ET-3, cortisol and renin.
[D]. Diabetes ELISA: insulin, proinsulin, leptin, resistin, c-peptide, adiponectin, ferritin, GLP-1 and anti-GAD.
[E]. Fertility ELISA: 5alpha-DHT, AMH, beta-hCG, DHEA, estradiol, FSH, progesterone, hPL, LH, pregnenolone, prolactin, SHBG and testosterone.
- Food Analytics and Safety ELISA Kits
Main categories include:
[A]. Allergens: Protein found in foods that may trigger an allergic reactions examples include: almonds, cashew, casein, egg white, fish, gluten, milk, peanut, mustard, soy, tropomyosin.
[B]. Mycotoxin: Classical example include: aflatoxin B1, aflatoxin M1, T2-toxin, fumonisin, deoxynivalenol (DON) and zearalenone.
[C]. Antibodics: Main groups are made up of penicillin, streptomycin, tetracyclins and chloramphenicol.
[D]. Vitamins: Examples include vitamin H (biotin), vitamin B12 and folic acid.
- Immunology and Cytokines ELISA Kits
Products can be grouped into cytokines, apoptosis, cell adhesion molecules (CAMs) and matrix metalloproteinases (MMPs).
[A]. Cytokines ELISA: Examples include: interleukins, chemokines, monokines, lymphokines, interferons and colony stimulating factors.
[B]. Apoptosis ELISA: Examples include: APRIL, annexin V, BAFF, bcl-2, apo-1, RBP4, TRAIL, p53.
[C]. CAMs ELISA: There are essentially four groups: cadherins, selectins, integrins and immunoglobulin superfamily (Ig) CAMs. Examples include: cadherin-11 (CDH11), cadherin-16 (CDH16), cadherin-17 (CDH17), E-cadherin (CDH1), H-cadherin (CDH13), N-cadherin (CDH2), P-cadherin (CDH3), E-selectin (CD62E), L-selectin (CD62L), P-selectin (CD62P), VCAM-1 (CD106), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50).
[D]. MMPs ELISA: These are grouped based on substrate preference and domain organisation. There are 5 groups which exist. Collagenase (e.g. MMP-1, MMP-8, MMP-13 and MMP-18), gelatinases (e.g. MMP-2 and MMP-9), stromelysins (e.g. MMP-3, MMP-10 and MMP-11), matrilysins (e.g. MMP-7 and MMP-26) and membrane type MMPs (e.g. MMP-14, MMP-15, MMP-16, MMP-17, MMP-24, MMP-25).
- Infectious Diseases ELISA Kits
Pathogenic micro-organisms such as bacteria, viruses, fungi and parasites are responsible for causing infectious diseases.
[A]. Bacteria diseases ELISA: Can be classified into three types of groupings. Vector borne diseases (e.g. borrelia), sexually transmitted diseases (e.g. syphilis and chlamydia) and vaccination management (e.g. diphtheria, tetanus and bordetella pertussis).
[B]. Viral diseases ELISA: These can be placed into two classes. Vector borne diseases (e.g. dengue fever, west nile and chikungunya) and vaccination management (e.g. mumps, measles and rubella).
[C]. Fungal diseases ELISA: Some of the popular fungal diseases include: aspergillus fumigatus, candida albicans, echinococcus granulosus and mycoplasma pneumoniae.
[D]. Parasite diseases ELISA: Common parasite diseases include: ascaris lumbricoides, chagas, dengue virus, entamoeba histolytica, giardia lamblia, leishmania, schistosoma, strongyloides.
[E]. TORCH diseases ELISA: The TORCH panel is made up of toxoplasmosis, rubella, cytomegalovirus (CMV) and herpes simplex virus (HSV).
- Salivary Diagnostics ELISA Kits
Saliva diagnostics is frequently being used to detect steroid hormone activity, some of the applications where it has been used include:
[A]. Endocrinology: measuring specific hormones which have been secreted by the endocrine system.
[B]. Analysing androgen status: Detecting free testosterone levels using saliva kits is an established for determining androgen levels in both males and females.
[C]. Cushing’s syndrome: Free cortisol measurement is recommended for patients suffering from Cushing’s syndrome.
[D]. Sports medicine: Many hormones are often analysed during sports sessions for example: DHEA and secretory IgA.
[E]. Anti-aging medicine: particular hormones that are measured include: estradiol, free progesterone and testosterone.
[F]. Sleep disorders: Usually the combined analysis of cortisol and melatonin have been instrumental in determining the circadian rhythms.
[G]. Stress research: Alpha amylase and free salivary cortisol are common biological parameters that are measured.
- Tumor Markers ELISA Kits
These biomarkers are often produced and secreted in response to benign and cancerous conditions. Some of the most common tumor markers include: AFP, CA-125, CA 15-3, CA 19-9, CA 72-4, CEA, CYFRA 21-1, PSA and NSE.
- Veterinary Diagnostics ELISA Kits
Our extensive portfolio includes many different animals such as: equine (horse), bovine (cattle), canine (dog), feline (cat), murine (rat/mouse) and many more.