GET A QUOTE

Milk ELISA Kit

  • Created on the 11 October, 2017.

BACKGROUND

Milk is usually processed into dairy products for example butter, cheese, yogurt, cream and ice-cream. It can also be found in many other found such as bread, breakfast cereals, processed meats, ready meals, soups, pizza, puddings and custards, sweets and cakes to juts name a few. A milk allergy, is very much like most of the other food allergies, it can occur during early infancy and cows’ milk protein allergy is regarded as the most frequent, however, many children tend to grow out of this allergy after the age of 5. Even low amounts of bovine milk can lead to allergic reactions and it may cause an anaphylactic shock in severe cases. An individual that it allergic to milk should therefore avoid milk or even the presence of hidden milk proteins present in foods such as convenience food, cookies, sausage and beverages. Approx. 80 % of bovine milk proteins are caseins and also beta-Lactoglobulin, represents a major allergen of whey (this represents an additional 10% of the total protein). In order to measure bovine milk in foodstuffs, a highly sensitive detection procedure is essential. This ELISA assay offers a very sensitive method for analysing milk based on NIST 1549 reference material, this protocol is validated to quantify casein and ß-lactoglobulin residues present in foods such as sausage, soy products, bread crumbs, cookies, chocolate, wine and orange juice.

INTENDED USE

Milk ELISA kit is designed for measuring quantitative amounts of bovine milk protein present in food (for example chocolate, bread crumbs, cookies, soy products, sausage, orange juice and wine). This assay has a minimum analytical sensitivity limit of 0.05 ppm.

SENSITIVITY

The minimum detection sensitivity level of bovine milk using this milk ELISA kit was 0.05 ppm. The standard assay range for this kit is 0.4 – 10.0 ppm.

REFERENCES

  1. A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk. Food Chem. (2014) 158: 445-8. Song F., et al.
  2. Short communication: Milk ELISA status for bovine leukosis virus infection is not associated with milk production in dairy cows. J Dairy Sci. (2011) 94 (10): 5062-4. Sorge U.S., et al.
  3. Role of maternal elimination diets and human milk IgA in the development of cow’s milk allergy in the infants. Clin Exp Allergy. (2014) 44 (1): 69-78. Järvinen K.M., et al.
  4. Human milk proteins may interfere in ELISA measurements of bovine beta-lactoglobulin in human milk. Acta Paediatr. (1996) 85 (5): 543-9. Bertino E., et al.
  5. Commercial Milk Enzyme-Linked Immunosorbent Assay (ELISA) Kit Reactivities to Purified Milk Proteins and Milk-Derived Ingredients. J Food Sci. (2016) 81 (7): T1871-8. Ivens K.O., et al.

ADDITIONAL INFORMATION

  • Full Name: Milk ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.05 ppm

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford