Mouse VEGF ELISA Kit (Vascular Endothelial Growth Factor)

Full Name: Mouse VEGF ELISA Kit (Vascular Endothelial Growth Factor)
Reactivity: Mouse
Sample Type: EDTA-Plasma, Serum, Cell Supernatant Media
Sensitivity: 11.0 pg/ml

BACKGROUND

In both the foetal and adulthood, vascular endothelial growth factor (VEGF or VEGF-A) is the mediator of angiogenesis and vasculogenesis. It belongs to the PDGF family, which is distinguished by its eight conserved cysteine residues arranged in a cystine knot shape and its ability to form anti-parallel disulfide-linked dimers. VEGF165, followed by VEGF121 and VEGF189, seems to be the most prevalent and powerful isoform in humans. Mice may display the similar pattern. Apart from VEGF120 and VEGF121, these isoforms have fundamental heparin-binding domains and are not easily diffusible, which result in their biological activities being restricted to a few sites. VEGFs exert their effects by binding to four membrane-spanning receptors, VEGFR1-4, which are also known as flk1/flk2.

The binding of VEGF ligands to these receptors results in activation of downstream signalling pathways that stimulate gene transcription and protein synthesis. In addition to endothelial cells, fibroblasts (skin cells) express these receptors and bind VEGF. VEGFs also bind to and stimulate the activity of the endothelin-B receptor (ET-B). The ET-B is a receptor that is produced by endothelial cells, which stimulates vasoconstriction, inhibits vascular permeability and affects the balance between salt and water retention. This receptor is involved in mediating many biological effects of VEGF.

INTENDED USE

Mouse VEGF ELISA kit is designed for measuring quantitative levels of vascular endothelial growth factor (VEGF) using mouse serum, EDTA-plasma and cell supernatant media. This assay has a minimum sensitivity detection limit of 11.0 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti-mouse-VEGF (V-N) rabbit IgG.
• Labelled Antibody (30x Concentrated).
• Standards (0-8): Concentration 0, 62.5, 125, 250, 500, 1,000, 2,000, 4,000 pg/ml
• EIA Buffer.
• Solution For Labelled Antibody.
• TMB Chromogen Solution.
• Stop Solution.
• Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of vascular endothelial growth factor using current mouse VEGF ELISA kit is 11.0 pg/ml. The dynamic range for this assay is 62.5 – 4,000.0 pg/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 95.8 – 103.8%
– Added Recovery Assay: 81.13 – 101.11%
– Intra – Assay: 2.8 – 10.2%
– Inter – Assay: 4.1 – 6.4%
– Linearity: 111.0 – 123.0%
– Specificity (Cross Reaction): Mouse VEGF 164 (100.0%), Human VEGF 165 (≦0.1%)
– Sensitivity: 10.98 pg/ml

REFERENCES

1. Extracellular regulation of VEGF: isoforms, proteolysis, and vascular patterning. Cytokine Growth Factor Rev. (2014) 25 (1): 1-19. Review. Vempati P., et al.
2. Adipose_VEGF Links the White-to-Brown Fat Switch With Environmental, Genetic, and Pharmacological Stimuli in Male Mice. Endocrinology. (2015) 156 (6): 2059-73. During M.J., et al.
3. Loss of autocrine endothelial-derived-VEGF significantly reduces hemangiosarcoma development in conditional p53-deficient mice. Cell Cycle. (2014) 13 (9): 1501-7. Farhang Ghahremani M., et al.
4. Induction of aberrant vascular growth, but not of normal angiogenesis, by cell-based expression of different doses of human and mouse VEGF is species-dependent. Hum Gene Ther Methods. (2013) 24 (1): 28-37. Mujagic E., et al.
5. Therapeutic potential of VEGF and VEGF-derived peptide in peripheral neuropathies. Neuroscience. (2013) 244: 77-89. Verheyen A., et al.

OTHER RELATED ELISA KITS

• VEGF ELISA Kit (Rat)
• VEGF ELISA Kit
• TGF-Alpha ELISA Kit