TFAM ELISA Kit (Transcription Factor A, Mitochondrial)

Full Name: TFAM ELISA Kit (Transcription Factor A, Mitochondrial)
Reactivity: Human
Sample Type: Tissue Homogenates, Biological Fluids, Plasma, Serum
Sensitivity: 18.75 pg/ml

INTRODUCTION

TFAM is described as a one of the core mitochondrial transcription factors. It plays an important role in the recruitment of mitochondrial RNA polymerase and the T2BM transcription factor to initial and activate the transcription process. One of the main roles is to coat and pack mtDNA into nucleoids by forming a U-turn on the mtDNA and creating cross strand interactions. It is also plays a role in the assembly of mitochondrial ribosomes. It can bind to RNA on the translating ribosome and either prevent it from exiting, stabilize it, or enhance its translation process.

TFAM is involved in a crease in mitochondrial membrane potential called mitochondrial cristae biogenesis. It binds to the proton channel of the inner membrane, this increases the amount of free protons within mitochondria and an increase in membrane potential. This causes release of ATP by opening a channel between two subunits of ClpB protein that are normally attached to each other, which allows for the release of ATP. Mitochondrial cristae are small folds in the mitochondrial membrane that have three membranes inside them and allow for diffusion of ions and molecules. Mitochondria need to be able to create these folds so they can expand their surface area when organelles divide. This process is called mitochondrial cristae biogenesis or MCP formation.

TFAM is a member of the high mobility group (HMG-box) family of proteins. It can function in unwinding, bending and binding mtDNA without any sequence specificity. One of the main functions is in maintaining and organising mtDNA into nucleoids. Furthermore, it is also essential for mitochondrial transcription initiation process.

INTENDED USE

Human TFAM ELISA kit can be used for measuring amounts of transcription factor A, mitochondrial (TFAM) present in human samples of serum, tissue homogenates, other biological fluids and plasma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- TFAM antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this TFAM ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TFAM, no cross reactivity or interference between TFAM and analogues was detected.
– Recovery: Serum (88 – 103%), EDTA Plasma (88 – 102%), Heparin Plasma (85 – 102%).
– Linearity: Serum (86 – 105%), EDTA Plasma (88 – 100%), Heparin Plasma (84 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Mitochondrial TFAM as a Signaling Regulator between Cellular Organelles: A Perspective on Metabolic Diseases. Diabetes Metab J. (2021) 45 (6): 853-865. Koh J.H., et al.
2. The Role of Exercise and TFAM in Preventing Skeletal Muscle Atrophy. J Cell Physiol. (2017) 232 (9): 2348-2358. Theilen N.T., et al.
3. Mitochondrial transcription factor A(TFAM) shapes metabolic and invasion gene signatures in melanoma. Sci Rep. (2018) 8 (1): 14190. Araujo L.F., et al.
4. High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo. Life Sci Alliance. (2021) 4 (11): e202101034. Bonekamp N.A., et al.

OTHER RELATED ELISA KITS

• Protein S100-B ELISA Kit
• CD59 ELISA Kit
• VDBP ELISA Kit