Human strongyloides IgG/IgM ELISA kit is intended for detecting in-vitro quantitative amounts of IgG and IgM antibodies against strongyloides (strongyloides-IgG/IgM) using human plasma and serum. This assay has a minimum analytical sensitivity limit of diagn. 87.9%.
Strongyloides (common name threadworm) is a human parasite roundworm which is responsible for strongyloidiasis disease. S. stercoralis is one of the most clinically important pathogenic species in humans and some of the distinctive characteristics of this parasite include ability to replicate and persist within a host for decades without any symptoms and having a potential to causing life-threatening infections within an immunocompromised host. Some of the symptoms of the infection are abdominal cramping, watery diarrhea and urticarial rash. During uncomplicated chronic infections, the larvae is able to migrate to the skin where it is able to cause cutaneous strongyloidiasis (also called larva currens). The adult parasitic stage occurs in the mucosa tunnels of the small intestine. Locating juvenile larvae (filariform or rhabditiform) can be confirmed using recent stool samples for analysis, other methods utilised include culturing fecal samples on agar plates, direct fecal smears, duodenal fumigation and serodiagnosis through ELISA.
STRONGYLOIDES IGG/IGM ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Strongyloides Coated Microplate.
- Positive Control (Cal. C).
- Cut-off Control (Cal. B).
- Negative Control (Cal. A).
- Strongyloides Conjugate.
- Sample Diluent.
- TMB Substrate Solution.
- Washing Buffer (20x Conc.).
- Stop Solution.
The minimum detection sensitivity level of IgM and IgG antibodies to strongyloides (strongyloides IgG and IgM) using current human strongyloides-IgG/IgM ELISA kit was diagn. 87.9%. The dynamic range for this assay is to a specific cut-off point.
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- Antibodies to Strongyloides stercoralis larval surface antigens in chronic strongyloidiasis. Lab Invest. (1982) 47 (1): 87-90. Genta R.M. and Weil G.J.
- Intestinal nematodes, Toxocara infection, and pyogenic liver abscess in children: a possible association. J Trop Pediatr. (2000) 46 (3): 167-72. Moreira-Silva S.F. and Pereira F.E.
- Immunological features in different clinical forms of strongyloidiasis. Trans R Soc Trop Med Hyg. (1983) 77 (3): 346-9. Carvalho E.M., et al.
- Full Name: Strongyloides IgG/IgM ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 87.9%