Strongyloides IgG/IgM ELISA Kit

Full Name: Strongyloides IgG/IgM ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 87.9%

BACKGROUND

Strongyloides (common name threadworm) is a human parasite roundworm which is responsible for strongyloidiasis disease. S. stercoralis is one of the most clinically important pathogenic species in humans and some of the distinctive characteristics of this parasite include ability to replicate and persist within a host for decades without any symptoms and having a potential to causing life-threatening infections within an immunocompromised host.

During uncomplicated chronic infections, the strongyloides larvae is able to migrate to the skin where it is able to cause cutaneous strongyloidiasis (also called larva currens). The adult parasitic stage occurs in the mucosa tunnels of the small intestine. Locating juvenile larvae (filariform or rhabditiform) can be confirmed using recent stool samples for analysis, other methods utilised include culturing fecal samples on agar plates, direct fecal smears, duodenal fumigation and serodiagnosis through ELISA.

INTENDED USE

Human strongyloides IgG/IgM ELISA kit is intended for detecting in-vitro amounts of IgG and IgM antibodies against strongyloides (strongyloides-IgG/IgM) using human plasma and serum. This assay has a minimum analytical sensitivity limit of diagn. 87.9%.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Strongyloides Coated Microplate.
• Positive Control (Cal. C).
• Cut-off Control (Cal. B).
• Negative Control (Cal. A).
  
• Strongyloides Conjugate.
• Sample Diluent.
• TMB Substrate Solution.
• Washing Buffer (20x Conc.).
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of IgM and IgG antibodies to strongyloides (strongyloides IgG and IgM) using current strongyloides IgG/IgM ELISA kit was diagn. 87.9%. The dynamic range for this assay is to a specific cut-off point.

REFERENCES

1. Innate and adaptive immunity to the nematode Strongyloides stercoralis in a mouse model. Immunol Res. (2011) 51 (2-3): 205-14. Review. Bonne-Année S., et al.
2. Antibodies to Strongyloides_stercoralis larval surface antigens in chronic strongyloidiasis. Lab Invest. (1982) 47 (1): 87-90. Genta R.M. and Weil G.J.
3. Intestinal nematodes, Toxocara infection, and pyogenic liver abscess in children: a possible association. J Trop Pediatr. (2000) 46 (3): 167-72. Moreira-Silva S.F. and Pereira F.E.
4. Immunological features in different clinical forms of strongyloidiasis. Trans R Soc Trop Med Hyg. (1983) 77 (3): 346-9. Carvalho E.M., et al.

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