Rat IL-6 ELISA Kit (Interleukin 6)

INTENDED USE

Rat IL-6 ELISA kit is intended for analysing concentrations of interleukin 6 (IL-6, IL6, BSF2, HGF, HSF, IFNB2, CDF) present in rat plasma, serum, biological fluids and tissue homogenates. This assay has a minimum sensitivity detection limit of 37.5 pg/ml.

INTRODUCTION

Interleukin 6 (IL-6) is a major mediator of inflammation and participates in the initiation, amplification and perpetuation of numerous inflammatory responses. Human neutrophils produce IL-6 during phagocytosis to facilitate their immune response against invading organisms. It has been established that the presence of Gram negative bacteria’s lipopolysaccharide (LPS) molecules on the surface membrane of these cells controls production. In gerbil granulocyte macrophages LPS increased IL-6 production. IL-6 is a key cytokine in immune system inflammation. Its downstream target gene, TGFβ1 was found to be up regulated during injury or infection. TGFB1 inhibits the function of pro-inflammatory molecules such as NFκB and COX2 by controlling their expression levels at the transcriptional level.

The sequence of the IL-6 gene produces a precursor protein composed of 211 amino acid residues. Upon cleavage, the mature protein is left with 187 amino acid residues and a hydrophobic signal peptide. A comparison of rat and human protein sequences reveals an approximate identity of 39%, while mouse and rat exhibit a higher identity rate of 87% and 86%, respectively. Circulating levels of IL-6 are raised when the body is exposed to particular substances or drugs, such as after sustaining a spinal cord injury. The amount then gradually falls over time as a result of scarring that results from the damage or lessening inflammation in the injured area.

IL-6 is created by various cells in response to several different stimuli such as antigenic or mitogenic activation, lipopolysaccharides, calcium ionophores, cytokines and viruses. Its production is triggered by these signals. It’s presence is being observed across lymphoid and non-lymphoid cells in both normal and transformed states. It has been found that an overproduction of serum levels could be a symptom of multiple diseases such as infections, autoimmune issues, injury trauma or even cancer.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with IL-6
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

The following rat interleukin 6 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml.
Reactivity: Rat
Sensitivity: 37.5 pg/ml
Range: 62.5 – 4000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-6, no cross reactivity or interference between IL-6 and analogues was detected.
– Recovery: Serum (87 – 105%), EDTA Plasma (85 – 103%), Heparin Plasma (94 – 105%).
– Linearity: Serum (86 – 103%), EDTA Plasma (92 – 98%), Heparin Plasma (81 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. IL-6 increases airway resistance in the rat. (2010) 51 (3): 266-73. Rubini A.
2. IL-6-induced skeletal muscle atrophy. J Appl Physiol (1985). (2005) 98 (3): 911-7. Haddad F., et al.
3. Absence of expression of interleukin-6 (IL-6) mRNA in regenerating rat liver. FEBS Lett. (1993) 315 (2): 159-62. Scotté M., et al.

ADDITIONAL INFORMATION

• Full Name: Rat IL-6 ELISA Kit (Interleukin 6)
• Reactivity: Rat
• Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
• Sensitivity: 37.5 pg/ml

OTHER RELATED ELISA KITS

• Rat IL-10 ELISA Kit
• Mouse TNF Alpha ELISA Kit
• Mouse IL-23 ELISA Kit

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